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Isolation of Microglia from Mouse or Human Tissue.

Publication ,  Journal Article
Bordt, EA; Block, CL; Petrozziello, T; Sadri-Vakili, G; Smith, CJ; Edlow, AG; Bilbo, SD
Published in: STAR protocols
June 2020

Microglia are the innate immune cells of the central nervous system. Although numerous methods have been developed to isolate microglia from the brain, the method of dissociation and isolation can have a profound effect on the function of these highly dynamic cells. Here, we present an optimized protocol to isolate CD11b+ cells (microglia) from mouse or human brain tissue using magnetic bead columns. Isolated microglia can be used to model diseases with neuroinflammatory components for potential therapeutic discoveries. For complete details on the use and execution of this protocol, please refer to Hanamsagar et al. (2017), Rivera et al. (2019), and Edlow et al. (2019).

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Published In

STAR protocols

DOI

EISSN

2666-1667

ISSN

2666-1667

Publication Date

June 2020

Volume

1

Issue

1

Start / End Page

100035

Related Subject Headings

  • Microglia
  • Mice
  • Humans
  • Cell Separation
  • CD11b Antigen
  • Animals
 

Citation

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Bordt, E. A., Block, C. L., Petrozziello, T., Sadri-Vakili, G., Smith, C. J., Edlow, A. G., & Bilbo, S. D. (2020). Isolation of Microglia from Mouse or Human Tissue. STAR Protocols, 1(1), 100035. https://doi.org/10.1016/j.xpro.2020.100035
Bordt, Evan A., Carina L. Block, Tiziana Petrozziello, Ghazaleh Sadri-Vakili, Caroline J. Smith, Andrea G. Edlow, and Staci D. Bilbo. “Isolation of Microglia from Mouse or Human Tissue.STAR Protocols 1, no. 1 (June 2020): 100035. https://doi.org/10.1016/j.xpro.2020.100035.
Bordt EA, Block CL, Petrozziello T, Sadri-Vakili G, Smith CJ, Edlow AG, et al. Isolation of Microglia from Mouse or Human Tissue. STAR protocols. 2020 Jun;1(1):100035.
Bordt, Evan A., et al. “Isolation of Microglia from Mouse or Human Tissue.STAR Protocols, vol. 1, no. 1, June 2020, p. 100035. Epmc, doi:10.1016/j.xpro.2020.100035.
Bordt EA, Block CL, Petrozziello T, Sadri-Vakili G, Smith CJ, Edlow AG, Bilbo SD. Isolation of Microglia from Mouse or Human Tissue. STAR protocols. 2020 Jun;1(1):100035.
Journal cover image

Published In

STAR protocols

DOI

EISSN

2666-1667

ISSN

2666-1667

Publication Date

June 2020

Volume

1

Issue

1

Start / End Page

100035

Related Subject Headings

  • Microglia
  • Mice
  • Humans
  • Cell Separation
  • CD11b Antigen
  • Animals