High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products.
Publication
, Chapter
Gamble, D; Hum, YF; Jinks-Robertson, S
2021
Mitotic double-strand breaks (DSBs) are repaired by recombination with a homologous donor duplex. This process involves the exchange of single DNA strands between the broken molecule and the repair template, giving rise to regions of heteroduplex DNA (hetDNA). The creation of a defined DSB coupled with the use of a sequence-diverged repair template allows the fine-structure mapping of hetDNA through the sequencing of recombination products. A high-throughput method is described that capitalizes on the single-molecule real-time (SMRT) sequencing technology developed by PacBio. This method allows simultaneous analysis of the hetDNA contained within hundreds of recombination products.
Duke Scholars
DOI
Publication Date
2021
Volume
2153
Start / End Page
503 / 519
Related Subject Headings
- Single Molecule Imaging
- Saccharomyces cerevisiae
- Mitosis
- High-Throughput Nucleotide Sequencing
- Gene Conversion
- Developmental Biology
- DNA, Fungal
- DNA Breaks, Double-Stranded
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Gamble, D., Hum, Y. F., & Jinks-Robertson, S. (2021). High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products. (Vol. 2153, pp. 503–519). https://doi.org/10.1007/978-1-0716-0644-5_34
Gamble, Dionna, Yee Fang Hum, and Sue Jinks-Robertson. “High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products.,” 2153:503–19, 2021. https://doi.org/10.1007/978-1-0716-0644-5_34.
Gamble D, Hum YF, Jinks-Robertson S. High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products. In 2021. p. 503–19.
Gamble, Dionna, et al. High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products. Vol. 2153, 2021, pp. 503–19. Pubmed, doi:10.1007/978-1-0716-0644-5_34.
Gamble D, Hum YF, Jinks-Robertson S. High-Throughput Analysis of Heteroduplex DNA in Mitotic Recombination Products. 2021. p. 503–519.
DOI
Publication Date
2021
Volume
2153
Start / End Page
503 / 519
Related Subject Headings
- Single Molecule Imaging
- Saccharomyces cerevisiae
- Mitosis
- High-Throughput Nucleotide Sequencing
- Gene Conversion
- Developmental Biology
- DNA, Fungal
- DNA Breaks, Double-Stranded
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology