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Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis.

Publication ,  Journal Article
Nomura, K; Mecey, C; Lee, Y-N; Imboden, LA; Chang, JH; He, SY
Published in: Proceedings of the National Academy of Sciences of the United States of America
June 2011

Innate immunity in plants can be triggered by microbe- and pathogen-associated molecular patterns. The pathogen-associated molecular pattern-triggered immunity (PTI) is often suppressed by pathogen effectors delivered into the host cell. Plants can overcome pathogen suppression of PTI and reestablish pathogen resistance through effector-triggered immunity (ETI). An unanswered question is how plants might overcome pathogen-suppression of PTI during ETI. Findings described in this paper suggest a possible mechanism. During Pseudomonas syringae pathovar tomato (Pst) DC3000 infection of Arabidopsis, a host ADP ribosylation factor guanine nucleotide exchange factor, AtMIN7, is destabilized by the pathogen effector HopM1 through the host 26S proteasome. In this study, we discovered that AtMIN7 is required for not only PTI, consistent with the notion that Pst DC3000 degrades AtMIN7 to suppress PTI, but also ETI. The AtMIN7 level in healthy plants is low, but increases posttranscriptionally in response to activation of PTI. Whereas DC3000 infection led to degradation of AtMIN7, activation of ETI by three different effectors, AvrRpt2, AvrPphB, and HopA1, in Col-0 plants blocks the ability of Pst DC3000 to destabilize AtMIN7. Further analyses of bacterial translocation of HopM1 and AtMIN7 stability in HopM1 transgenic plants show that ETI prevents HopM1-mediated degradation of AtMIN7 inside the plant cell. Both AtMIN7 and HopM1 are localized to the trans-Golgi network/early endosome, a subcellular compartment that is not previously known to be associated with bacterial pathogenesis in plants. Thus, blocking pathogen degradation of trans-Golgi network/early endosome-associated AtMIN7 is a critical part of the ETI mechanism to counter bacterial suppression of PTI.

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Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

June 2011

Volume

108

Issue

26

Start / End Page

10774 / 10779

Related Subject Headings

  • Pseudomonas syringae
  • Hydrolysis
  • Host-Pathogen Interactions
  • Guanine Nucleotide Exchange Factors
  • Arabidopsis Proteins
  • Arabidopsis
 

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Nomura, K., Mecey, C., Lee, Y.-N., Imboden, L. A., Chang, J. H., & He, S. Y. (2011). Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 108(26), 10774–10779. https://doi.org/10.1073/pnas.1103338108
Nomura, Kinya, Christy Mecey, Young-Nam Lee, Lori Alice Imboden, Jeff H. Chang, and Sheng Yang He. “Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America 108, no. 26 (June 2011): 10774–79. https://doi.org/10.1073/pnas.1103338108.
Nomura K, Mecey C, Lee Y-N, Imboden LA, Chang JH, He SY. Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 2011 Jun;108(26):10774–9.
Nomura, Kinya, et al. “Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 26, June 2011, pp. 10774–79. Epmc, doi:10.1073/pnas.1103338108.
Nomura K, Mecey C, Lee Y-N, Imboden LA, Chang JH, He SY. Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 2011 Jun;108(26):10774–10779.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

June 2011

Volume

108

Issue

26

Start / End Page

10774 / 10779

Related Subject Headings

  • Pseudomonas syringae
  • Hydrolysis
  • Host-Pathogen Interactions
  • Guanine Nucleotide Exchange Factors
  • Arabidopsis Proteins
  • Arabidopsis