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Conversion of compatible plant-pathogen interactions into incompatible interactions by expression of the Pseudomonas syringae pv. syringae 61 hrmA gene in transgenic tobacco plants.

Publication ,  Journal Article
Shen, S; Li, Q; He, SY; Barker, KR; Li, D; Hunt, AG
Published in: The Plant journal : for cell and molecular biology
July 2000

The hrmA gene from Pseudomonas syringae pv. syringae has previously been shown to confer avirulence on the virulent bacterium P. syringae pv. tabaci in all examined tobacco cultivars. We expressed this gene in tobacco plants under the control of the tobacco Delta0. 3 TobRB7 promoter, which is induced upon nematode infection in tobacco roots (Opperman et al. 1994, Science, 263, 221-223). A basal level of hrmA expression in leaves of transgenic plants activated the expression of pathogenesis-related genes, and the transgenic plants exhibited high levels of resistance to multiple pathogens: tobacco vein mottling virus, tobacco etch virus, black shank fungus Phytophthora parasitica, and wild fire bacterium Pseudomonas syringae pv. tabaci. However, the hrmA transgenic plants were not significantly more resistant to root-knot nematodes. Our results suggest a potential use of controlled low-level expression of bacterial avr genes, such as hrmA, in plants to generate broad-spectrum resistance to bacterial, fungal and viral pathogens.

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Published In

The Plant journal : for cell and molecular biology

DOI

EISSN

1365-313X

ISSN

0960-7412

Publication Date

July 2000

Volume

23

Issue

2

Start / End Page

205 / 213

Related Subject Headings

  • Virulence
  • Pseudomonas
  • Promoter Regions, Genetic
  • Plants, Toxic
  • Plants, Genetically Modified
  • Plant Leaves
  • Plant Biology & Botany
  • Nicotiana
  • Nematoda
  • Immunity, Innate
 

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Shen, S., Li, Q., He, S. Y., Barker, K. R., Li, D., & Hunt, A. G. (2000). Conversion of compatible plant-pathogen interactions into incompatible interactions by expression of the Pseudomonas syringae pv. syringae 61 hrmA gene in transgenic tobacco plants. The Plant Journal : For Cell and Molecular Biology, 23(2), 205–213. https://doi.org/10.1046/j.1365-313x.2000.00772.x
Shen, S., Q. Li, S. Y. He, K. R. Barker, D. Li, and A. G. Hunt. “Conversion of compatible plant-pathogen interactions into incompatible interactions by expression of the Pseudomonas syringae pv. syringae 61 hrmA gene in transgenic tobacco plants.The Plant Journal : For Cell and Molecular Biology 23, no. 2 (July 2000): 205–13. https://doi.org/10.1046/j.1365-313x.2000.00772.x.
Shen S, Li Q, He SY, Barker KR, Li D, Hunt AG. Conversion of compatible plant-pathogen interactions into incompatible interactions by expression of the Pseudomonas syringae pv. syringae 61 hrmA gene in transgenic tobacco plants. The Plant journal : for cell and molecular biology. 2000 Jul;23(2):205–13.
Shen, S., et al. “Conversion of compatible plant-pathogen interactions into incompatible interactions by expression of the Pseudomonas syringae pv. syringae 61 hrmA gene in transgenic tobacco plants.The Plant Journal : For Cell and Molecular Biology, vol. 23, no. 2, July 2000, pp. 205–13. Epmc, doi:10.1046/j.1365-313x.2000.00772.x.
Shen S, Li Q, He SY, Barker KR, Li D, Hunt AG. Conversion of compatible plant-pathogen interactions into incompatible interactions by expression of the Pseudomonas syringae pv. syringae 61 hrmA gene in transgenic tobacco plants. The Plant journal : for cell and molecular biology. 2000 Jul;23(2):205–213.
Journal cover image

Published In

The Plant journal : for cell and molecular biology

DOI

EISSN

1365-313X

ISSN

0960-7412

Publication Date

July 2000

Volume

23

Issue

2

Start / End Page

205 / 213

Related Subject Headings

  • Virulence
  • Pseudomonas
  • Promoter Regions, Genetic
  • Plants, Toxic
  • Plants, Genetically Modified
  • Plant Leaves
  • Plant Biology & Botany
  • Nicotiana
  • Nematoda
  • Immunity, Innate