Skip to main content
Journal cover image

Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7.

Publication ,  Journal Article
Thilmony, R; Underwood, W; He, SY
Published in: The Plant journal : for cell and molecular biology
April 2006

Pseudomonas syringae pv. tomato DC3000 (Pst) is a virulent pathogen that causes disease on tomato and Arabidopsis. The type III secretion system (TTSS) plays a key role in pathogenesis by translocating virulence effectors from the bacteria into the plant host cell, while the phytotoxin coronatine (COR) contributes to virulence and disease symptom development. Recent studies suggest that both the TTSS and COR are involved in the suppression of host basal defenses. However, little is known about the interplay between the host gene expression changes associated with basal defenses and the virulence activities of the TTSS and COR during infection. In this study, we used the Affymetrix full genome chip to determine the Arabidopsis transcriptome associated with basal defense to Pst DC3000 hrp mutants and the human pathogenic bacterium Escherichia coli O157:H7. We then used Pst DC3000 virulence mutants to characterize Arabidopsis transcriptional responses to the action of hrp-regulated virulence factors (e.g. TTSS and COR) during bacterial infection. Additionally, we used bacterial fliC mutants to assess the role of the pathogen-associated molecular pattern flagellin in induction of basal defense-associated transcriptional responses. In total, our global gene expression analysis identified 2800 Arabidopsis genes that are reproducibly regulated in response to bacterial pathogen inoculation. Regulation of these genes provides a molecular signature for Arabidopsis basal defense to plant and human pathogenic bacteria, and illustrates both common and distinct global virulence effects of the TTSS, COR, and possibly other hrp-regulated virulence factors during Pst DC3000 infection.

Duke Scholars

Published In

The Plant journal : for cell and molecular biology

DOI

EISSN

1365-313X

ISSN

0960-7412

Publication Date

April 2006

Volume

46

Issue

1

Start / End Page

34 / 53

Related Subject Headings

  • Virulence Factors
  • Transcription Factors
  • Pseudomonas syringae
  • Plant Diseases
  • Plant Biology & Botany
  • Oxylipins
  • Mutation
  • Immunity, Innate
  • Genome, Plant
  • Gene Expression Regulation, Plant
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Thilmony, R., Underwood, W., & He, S. Y. (2006). Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7. The Plant Journal : For Cell and Molecular Biology, 46(1), 34–53. https://doi.org/10.1111/j.1365-313x.2006.02725.x
Thilmony, Roger, William Underwood, and Sheng Yang He. “Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7.The Plant Journal : For Cell and Molecular Biology 46, no. 1 (April 2006): 34–53. https://doi.org/10.1111/j.1365-313x.2006.02725.x.
Thilmony, Roger, et al. “Genome-wide transcriptional analysis of the Arabidopsis thaliana interaction with the plant pathogen Pseudomonas syringae pv. tomato DC3000 and the human pathogen Escherichia coli O157:H7.The Plant Journal : For Cell and Molecular Biology, vol. 46, no. 1, Apr. 2006, pp. 34–53. Epmc, doi:10.1111/j.1365-313x.2006.02725.x.
Journal cover image

Published In

The Plant journal : for cell and molecular biology

DOI

EISSN

1365-313X

ISSN

0960-7412

Publication Date

April 2006

Volume

46

Issue

1

Start / End Page

34 / 53

Related Subject Headings

  • Virulence Factors
  • Transcription Factors
  • Pseudomonas syringae
  • Plant Diseases
  • Plant Biology & Botany
  • Oxylipins
  • Mutation
  • Immunity, Innate
  • Genome, Plant
  • Gene Expression Regulation, Plant