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Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media.

Publication ,  Journal Article
Wang, X; Hodgkinson, CP; Dzau, VJ
Published in: Methods Mol Biol
2021

There are currently no effective treatments to regenerate the heart after cardiac injury. Following cardiac injury, heart muscle cells, also known as cardiomyocytes, die in large numbers. The adult mammalian heart does not have the ability to replace these dead cardiomyocytes. In their place, fibroblasts invade the injury zone and generate a scar. The scar impairs cardiac function. An important approach to cardiac regeneration is direct cardiac reprogramming, whereby cardiac fibroblasts within the scar are directly converted into functional cardiomyocytes. Several laboratories have achieved direct cardiac reprogramming via overexpression of the cardiac transcription factors. In contrast, we utilize a combination of four miRNAs (miR-1, miR-133, miR-208, miR-499) that we call miR Combo. One common issue regarding direct cardiac reprogramming strategies is low efficiency. Recently, we have demonstrated that the efficiency of direct cardiac reprogramming is enhanced in the chemically defined reprogramming media.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2021

Volume

2239

Start / End Page

47 / 59

Location

United States

Related Subject Headings

  • Transfection
  • Selenium
  • Regeneration
  • Real-Time Polymerase Chain Reaction
  • Myocytes, Cardiac
  • MicroRNAs
  • Mice
  • Fluorescent Antibody Technique
  • Flow Cytometry
  • Fibroblasts
 

Citation

APA
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ICMJE
MLA
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Wang, X., Hodgkinson, C. P., & Dzau, V. J. (2021). Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media. Methods Mol Biol, 2239, 47–59. https://doi.org/10.1007/978-1-0716-1084-8_4
Wang, Xiaowen, Conrad P. Hodgkinson, and Victor J. Dzau. “Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media.Methods Mol Biol 2239 (2021): 47–59. https://doi.org/10.1007/978-1-0716-1084-8_4.
Wang X, Hodgkinson CP, Dzau VJ. Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media. Methods Mol Biol. 2021;2239:47–59.
Wang, Xiaowen, et al. “Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media.Methods Mol Biol, vol. 2239, 2021, pp. 47–59. Pubmed, doi:10.1007/978-1-0716-1084-8_4.
Wang X, Hodgkinson CP, Dzau VJ. Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media. Methods Mol Biol. 2021;2239:47–59.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2021

Volume

2239

Start / End Page

47 / 59

Location

United States

Related Subject Headings

  • Transfection
  • Selenium
  • Regeneration
  • Real-Time Polymerase Chain Reaction
  • Myocytes, Cardiac
  • MicroRNAs
  • Mice
  • Fluorescent Antibody Technique
  • Flow Cytometry
  • Fibroblasts