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Immunomodulation of human CD8⁺ T cells by thymoglobulin in vitro.

Publication ,  Conference
Yu, L-Z; Fang, Y; Zhu, L; Sun, Z-G; Xu, H
Published in: Transplant Proc
May 2012

The major mechanism of thymoglobulin in inhibiting allograft rejection involves lymphocyte depletion. However, its role in modulating CD8(+) cells has remained unclear. This study evaluated the immunologic effects of thymoglobulin on human CD8(+) cells. Purified CD8(+) cells were pretreated with thymoglobulin followed by incubation for 72 hours. The culture supernates and cells were analyzed using real-time quantitative polymerase chain reaction (RT-PCR), multiplex cytokine detection assay, and flow cytometry. RT-PCR showed that thymoglobulin-treated CD8(+) cells up-regulated transcripts for CD25, CTLA-4, OX40, GITR, Foxp3, IFN-γ, interleukin (IL)-10, and IL-2 when compared with isotype control immunoglobulin (Ig). The expression for GITR and IL-2 transcripts was down-regulated at 72 hours after incubation. The release of IFN-γ, IL-10, and IL-2 from thymoglobulin-pretreated CD8(+) cells was detected using multiplex assay after 24-hour incubation, and these cytokine levels were decreased after incubation for 48 and 72 hours. Flow cytometry demonstrated up-regulation of CD69 and CD25 expression after treatment. The surface CTLA-4 and intracellular Foxp3 expression was not increased in thymoglobulin-treated cells. Our results demonstrated that thymoglobulin-treated CD8(+) cells up-regulate CD25, and multiple costimulatory molecules at the transcriptional level. The up-regulation of transcripts of immune regulatory cytokines accompanies the release of these cytokines. The unique effects of thymoglobulin on CD8(+) cells may be an important mechanism for its action in inducing immunosuppression and immunomodulation.

Duke Scholars

Published In

Transplant Proc

DOI

EISSN

1873-2623

Publication Date

May 2012

Volume

44

Issue

4

Start / End Page

1052 / 1054

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transcription, Genetic
  • Time Factors
  • Receptors, OX40
  • Real-Time Polymerase Chain Reaction
  • RNA, Messenger
  • Lectins, C-Type
  • Interleukin-2 Receptor alpha Subunit
  • Immunosuppressive Agents
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yu, L.-Z., Fang, Y., Zhu, L., Sun, Z.-G., & Xu, H. (2012). Immunomodulation of human CD8⁺ T cells by thymoglobulin in vitro. In Transplant Proc (Vol. 44, pp. 1052–1054). United States. https://doi.org/10.1016/j.transproceed.2012.03.027
Yu, L. -. Z., Y. Fang, L. Zhu, Z. -. G. Sun, and H. Xu. “Immunomodulation of human CD8⁺ T cells by thymoglobulin in vitro.” In Transplant Proc, 44:1052–54, 2012. https://doi.org/10.1016/j.transproceed.2012.03.027.
Yu L-Z, Fang Y, Zhu L, Sun Z-G, Xu H. Immunomodulation of human CD8⁺ T cells by thymoglobulin in vitro. In: Transplant Proc. 2012. p. 1052–4.
Yu, L. .. Z., et al. “Immunomodulation of human CD8⁺ T cells by thymoglobulin in vitro.Transplant Proc, vol. 44, no. 4, 2012, pp. 1052–54. Pubmed, doi:10.1016/j.transproceed.2012.03.027.
Yu L-Z, Fang Y, Zhu L, Sun Z-G, Xu H. Immunomodulation of human CD8⁺ T cells by thymoglobulin in vitro. Transplant Proc. 2012. p. 1052–1054.
Journal cover image

Published In

Transplant Proc

DOI

EISSN

1873-2623

Publication Date

May 2012

Volume

44

Issue

4

Start / End Page

1052 / 1054

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transcription, Genetic
  • Time Factors
  • Receptors, OX40
  • Real-Time Polymerase Chain Reaction
  • RNA, Messenger
  • Lectins, C-Type
  • Interleukin-2 Receptor alpha Subunit
  • Immunosuppressive Agents
  • Humans