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Sample preparation of the human TRPA1 ion channel for cryo-EM studies.

Publication ,  Chapter
Suo, Y; Lee, S-Y
2021

The transient receptor potential ankyrin 1 (TRPA1) ion channel is a member of the TRP channel family that is involved in sensing noxious stimuli that elicit pain and inflammation. Because of its critical physiological role and therapeutic importance, great efforts have been made to understand the structure and mechanism of TRPA1. Several human TRPA1 structures have been reported using single particle cryo-electron microscopy (cryo-EM) over the last 6 years. Here, we present a protocol for the heterologous expression, large-scale purification, and nanodisc reconstitution of the human TRPA1 channel for cryo-EM and biochemical studies.

Duke Scholars

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DOI

Publication Date

2021

Volume

653

Start / End Page

75 / 87

Related Subject Headings

  • Transient Receptor Potential Channels
  • TRPA1 Cation Channel
  • Pain
  • Humans
  • Cytoskeletal Proteins
  • Cryoelectron Microscopy
  • Biochemistry & Molecular Biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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ICMJE
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Suo, Y., & Lee, S.-Y. (2021). Sample preparation of the human TRPA1 ion channel for cryo-EM studies. (Vol. 653, pp. 75–87). https://doi.org/10.1016/bs.mie.2020.12.018
Suo, Yang, and Seok-Yong Lee. “Sample preparation of the human TRPA1 ion channel for cryo-EM studies.,” 653:75–87, 2021. https://doi.org/10.1016/bs.mie.2020.12.018.
Suo, Yang, and Seok-Yong Lee. Sample preparation of the human TRPA1 ion channel for cryo-EM studies. Vol. 653, 2021, pp. 75–87. Pubmed, doi:10.1016/bs.mie.2020.12.018.

DOI

Publication Date

2021

Volume

653

Start / End Page

75 / 87

Related Subject Headings

  • Transient Receptor Potential Channels
  • TRPA1 Cation Channel
  • Pain
  • Humans
  • Cytoskeletal Proteins
  • Cryoelectron Microscopy
  • Biochemistry & Molecular Biology
  • 0601 Biochemistry and Cell Biology