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Efficacy of UV-C Disinfection in Hyperbaric Chambers

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Warren, B; Masker, J; Brown, G; Gamez, I; Smith, B; Anderson, DJ; Turner, N
Published in: Infection Control & Hospital Epidemiology
October 2020

UV-C light reduces contamination of high-touch clinical surfaces. Few studies have tested the relative efficacy of UV-C devices in real-world clinical environments. We assessed the efficacy of the Tru-D (SmartUVC) and Moonbeam-3 UV-C (Diversey) devices at eradicating important clinical pathogens in 2 hyperbaric chambers at a tertiary-care hospital. Formica sheets were inoculated with 106–107 CFU of MRSA (USA300) or 104–105 CFU of (NAP1). Sheets were placed in 6 predetermined locations throughout the chambers. Two Moonbeam-3 UV-C devices were positioned in the center of each chamber and were run for 3-minute (per manufacturer’s instructions) and 5-minute cycles. One Tru-D was positioned in the center of the chamber and was run on the vegetative cycle for MRSA and the spore cycle for UV-C dosage was measured for both machines. Quantitative cultures were collected using Rodac plates with DE neutralizing agar and were incubated at 37C for 48 hours. was likewise plated onto sheep’s blood agar. We ran each combination of chamber, microbe, and UV-C device in triplicate for In total, 108 samples per species. For MRSA, the Tru-D vegetative cycle, the 5-minute Moonbeam cycle, and the 3-minute Moonbeam cycle resulted in average CFU log10 reductions of 7.02 (95% CI, 7.02–7.02), 6.99 (95% CI, 6.95–7.02), and 6.58 (95% CI, 6.37–6.79), respectively (Fig. 1). The Tru-D vegetative and 5-minute Moonbeam cycles were similarly effective ( > .99), and both were more effective than the 3-minute Moonbeam cycle ( < .001 and < .001, respectively). MRSA samples receiving direct UV-C exposure had significantly greater log10 reductions (6.95; 95% CI, 6.89–7.01) than did indirect exposure (6.67; 95% CI, 6.46–6.87; < .05) (Fig. 2). For , the Tru-D sporicidal, the 5-, and 3-minute Moonbeam cycles resulted in average CFU log10 reductions of 1.78 (95% CI, 1.43–2.12), 0.57 (95% CI, 0.33–0.81) and 0.64 (95% CI, 0.42–0.86), respectively (Fig. 1). Tru-D was significantly more effective than either the 3- or 5-minute Moonbeam cycles ( < .00). samples receiving direct UV-C exposure had higher dosage and significantly greater log10 reductions (1.34; 95% CI, 1.10–1.58) than did indirect exposure (0.58; 95% CI, 0.31–0.86; < .01) (Fig. 2). Use of the Tru-D vegetative cycle and the Moonbeam 3- and 5-minute cycles resulted in similar reductions in MRSA; both resulted in significantly greater reductions than the manufacturer’s recommended 3-minute Moonbeam cycle. Therefore, healthcare facilities should carefully evaluate manufacturer-recommended run times in their specific clinical setting. For the Tru-D sporicidal cycle was significantly more effective than either of the Moonbeam cycles, likely due to higher irradiation levels. As such, direct UV-C exposure resulted in greater average reductions than indirect exposure. None None

Duke Scholars

Published In

Infection Control &amp; Hospital Epidemiology

DOI

EISSN

1559-6834

ISSN

0899-823X

Publication Date

October 2020

Volume

41

Issue

S1

Start / End Page

s210 / s211

Publisher

Cambridge University Press (CUP)

Related Subject Headings

  • Epidemiology
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 11 Medical and Health Sciences
 

Citation

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Warren, B., Masker, J., Brown, G., Gamez, I., Smith, B., Anderson, D. J., & Turner, N. (2020). Efficacy of UV-C Disinfection in Hyperbaric Chambers. In Infection Control &amp; Hospital Epidemiology (Vol. 41, pp. s210–s211). Cambridge University Press (CUP). https://doi.org/10.1017/ice.2020.753
Warren, Bobby, Jason Masker, Gregory Brown, Isabella Gamez, Becky Smith, Deverick John Anderson, and Nicholas Turner. “Efficacy of UV-C Disinfection in Hyperbaric Chambers.” In Infection Control &amp; Hospital Epidemiology, 41:s210–11. Cambridge University Press (CUP), 2020. https://doi.org/10.1017/ice.2020.753.
Warren B, Masker J, Brown G, Gamez I, Smith B, Anderson DJ, et al. Efficacy of UV-C Disinfection in Hyperbaric Chambers. In: Infection Control &amp; Hospital Epidemiology. Cambridge University Press (CUP); 2020. p. s210–1.
Warren, Bobby, et al. “Efficacy of UV-C Disinfection in Hyperbaric Chambers.” Infection Control &amp; Hospital Epidemiology, vol. 41, no. S1, Cambridge University Press (CUP), 2020, pp. s210–11. Crossref, doi:10.1017/ice.2020.753.
Warren B, Masker J, Brown G, Gamez I, Smith B, Anderson DJ, Turner N. Efficacy of UV-C Disinfection in Hyperbaric Chambers. Infection Control &amp; Hospital Epidemiology. Cambridge University Press (CUP); 2020. p. s210–s211.
Journal cover image

Published In

Infection Control &amp; Hospital Epidemiology

DOI

EISSN

1559-6834

ISSN

0899-823X

Publication Date

October 2020

Volume

41

Issue

S1

Start / End Page

s210 / s211

Publisher

Cambridge University Press (CUP)

Related Subject Headings

  • Epidemiology
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
  • 11 Medical and Health Sciences