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Prevalence of hepatitis B antiviral drug resistance variants in North American patients with chronic hepatitis B not receiving antiviral treatment.

Publication ,  Journal Article
Lok, AS; Ganova-Raeva, L; Cloonan, Y; Punkova, L; Lin, H-HS; Lee, WM; Ghany, MG; Hepatitis B Research Network (HBRN),
Published in: J Viral Hepat
November 2017

Antiviral drug resistance hepatitis B virus (HBV) variants (HBV-DR) occur spontaneously in chronic hepatitis B (CHB) patients and after exposure to nucleos(t)ide analogues (NUCs). We determined the prevalence of HBV-DR variants among participants of the Hepatitis B Research Network (HBRN) Cohort Study conducted at 21 sites in the United States (US) and Canada. Samples obtained from 1342 CHB participants aged ≥18 years, and who were currently not receiving NUCs, were tested for HBV-DR variants by Sanger sequencing. In addition, next generation sequencing (NGS) was used to characterize HBV-DR variants from 66 participants with and 66 participants with no prior NUC exposure matched for HBV genotype and HBV DNA level. Half the participants were men, 75% Asian, 26% HBeAg positive. Primary HBV-DR variants were detected by Sanger sequencing in 16 (1.2%) participants: 2/142 (1.4%) with and 14/1200 (1.2%) without prior NUC exposure; only 1 of these 16 had a secondary variant. In total, 23 (1.7%) participants had secondary variants, including 1 with prior NUC experience. In the subset of 132 participants, NGS detected HBV-DR variants in a higher proportion of participants: primary variants in 18 (13.6%) (8 [12.1%] with, and 10 [15.2%] without prior NUC therapy) and secondary variants in 10 (7.6%) participants. Based on Sanger sequencing, prevalence of primary HBV-DR variants is low (1.2%) among adults with CHB in US/Canada. The similar low prevalence of HBV-DR variants in participants with and without NUC treatment suggests transmission of these variants is uncommon.

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Published In

J Viral Hepat

DOI

EISSN

1365-2893

Publication Date

November 2017

Volume

24

Issue

11

Start / End Page

1032 / 1042

Location

England

Related Subject Headings

  • Young Adult
  • Sequence Analysis, DNA
  • Prevalence
  • Population Surveillance
  • North America
  • Mutation
  • Middle Aged
  • Male
  • Humans
  • High-Throughput Nucleotide Sequencing
 

Citation

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MLA
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Lok, A. S., Ganova-Raeva, L., Cloonan, Y., Punkova, L., Lin, H.-H., Lee, W. M., … Hepatitis B Research Network (HBRN), . (2017). Prevalence of hepatitis B antiviral drug resistance variants in North American patients with chronic hepatitis B not receiving antiviral treatment. J Viral Hepat, 24(11), 1032–1042. https://doi.org/10.1111/jvh.12732
Lok, A. S., L. Ganova-Raeva, Y. Cloonan, L. Punkova, H. -. H. S. Lin, W. M. Lee, M. G. Ghany, and M. G. Hepatitis B Research Network (HBRN). “Prevalence of hepatitis B antiviral drug resistance variants in North American patients with chronic hepatitis B not receiving antiviral treatment.J Viral Hepat 24, no. 11 (November 2017): 1032–42. https://doi.org/10.1111/jvh.12732.
Lok AS, Ganova-Raeva L, Cloonan Y, Punkova L, Lin H-HS, Lee WM, et al. Prevalence of hepatitis B antiviral drug resistance variants in North American patients with chronic hepatitis B not receiving antiviral treatment. J Viral Hepat. 2017 Nov;24(11):1032–42.
Lok, A. S., et al. “Prevalence of hepatitis B antiviral drug resistance variants in North American patients with chronic hepatitis B not receiving antiviral treatment.J Viral Hepat, vol. 24, no. 11, Nov. 2017, pp. 1032–42. Pubmed, doi:10.1111/jvh.12732.
Lok AS, Ganova-Raeva L, Cloonan Y, Punkova L, Lin H-HS, Lee WM, Ghany MG, Hepatitis B Research Network (HBRN). Prevalence of hepatitis B antiviral drug resistance variants in North American patients with chronic hepatitis B not receiving antiviral treatment. J Viral Hepat. 2017 Nov;24(11):1032–1042.
Journal cover image

Published In

J Viral Hepat

DOI

EISSN

1365-2893

Publication Date

November 2017

Volume

24

Issue

11

Start / End Page

1032 / 1042

Location

England

Related Subject Headings

  • Young Adult
  • Sequence Analysis, DNA
  • Prevalence
  • Population Surveillance
  • North America
  • Mutation
  • Middle Aged
  • Male
  • Humans
  • High-Throughput Nucleotide Sequencing