Local nucleosome dynamics and eviction following a double-strand break are reversible by NHEJ-mediated repair in the absence of DNA replication.
We interrogated at nucleotide resolution the spatiotemporal order of chromatin changes that occur immediately following a site-specific double-strand break (DSB) upstream of the PHO5 locus and its subsequent repair by nonhomologous end joining (NHEJ). We observed the immediate eviction of a nucleosome flanking the break and the repositioning of adjacent nucleosomes away from the break. These early chromatin events were independent of the end-processing Mre11-Rad50-Xrs2 (MRX) complex and preceded the MRX-dependent broad eviction of histones and DNA end-resectioning that extends up to ∼8 kb away from the break. We also examined the temporal dynamics of NHEJ-mediated repair in a G1-arrested population. Concomitant with DSB repair by NHEJ, we observed the redeposition and precise repositioning of nucleosomes at their originally occupied positions. This re-establishment of the prelesion chromatin landscape suggests that a DNA replication-independent mechanism exists to preserve epigenome organization following DSB repair.
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- Nucleosomes
- DNA-Binding Proteins
- DNA Replication
- DNA Repair
- DNA End-Joining Repair
- DNA Breaks, Double-Stranded
- Bioinformatics
- 3105 Genetics
- 11 Medical and Health Sciences
- 06 Biological Sciences
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Nucleosomes
- DNA-Binding Proteins
- DNA Replication
- DNA Repair
- DNA End-Joining Repair
- DNA Breaks, Double-Stranded
- Bioinformatics
- 3105 Genetics
- 11 Medical and Health Sciences
- 06 Biological Sciences