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Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5.

Publication ,  Journal Article
Coyne, CB; Shen, L; Turner, JR; Bergelson, JM
Published in: Cell Host Microbe
September 13, 2007

The major group B coxsackievirus (CVB) receptor is a component of the epithelial tight junction (TJ), a protein complex that regulates the selective passage of ions and molecules across the epithelium. CVB enters polarized epithelial cells from the TJ, causing a transient disruption of TJ integrity. Here we show that CVB does not induce major reorganization of the TJ, but stimulates the specific internalization of occludin-a TJ integral membrane component-within macropinosomes. Although occludin does not interact directly with virus, depletion of occludin prevents CVB entry into the cytoplasm and inhibits infection. Both occludin internalization and CVB entry require caveolin but not dynamin; both are blocked by inhibitors of macropinocytosis and require the activity of Rab34, Ras, and Rab5, GTPases known to regulate macropinocytosis. Thus, CVB entry depends on occludin and occurs by a process that combines aspects of caveolar endocytosis with features characteristic of macropinocytosis.

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Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

September 13, 2007

Volume

2

Issue

3

Start / End Page

181 / 192

Location

United States

Related Subject Headings

  • ras GTPase-Activating Proteins
  • rab5 GTP-Binding Proteins
  • rab GTP-Binding Proteins
  • Virus Internalization
  • Tight Junctions
  • Occludin
  • Membrane Proteins
  • Immunology
  • Humans
  • Epithelial Cells
 

Citation

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Coyne, C. B., Shen, L., Turner, J. R., & Bergelson, J. M. (2007). Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5. Cell Host Microbe, 2(3), 181–192. https://doi.org/10.1016/j.chom.2007.07.003
Coyne, Carolyn B., Le Shen, Jerrold R. Turner, and Jeffrey M. Bergelson. “Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5.Cell Host Microbe 2, no. 3 (September 13, 2007): 181–92. https://doi.org/10.1016/j.chom.2007.07.003.
Coyne CB, Shen L, Turner JR, Bergelson JM. Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5. Cell Host Microbe. 2007 Sep 13;2(3):181–92.
Coyne, Carolyn B., et al. “Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5.Cell Host Microbe, vol. 2, no. 3, Sept. 2007, pp. 181–92. Pubmed, doi:10.1016/j.chom.2007.07.003.
Coyne CB, Shen L, Turner JR, Bergelson JM. Coxsackievirus entry across epithelial tight junctions requires occludin and the small GTPases Rab34 and Rab5. Cell Host Microbe. 2007 Sep 13;2(3):181–192.
Journal cover image

Published In

Cell Host Microbe

DOI

EISSN

1934-6069

Publication Date

September 13, 2007

Volume

2

Issue

3

Start / End Page

181 / 192

Location

United States

Related Subject Headings

  • ras GTPase-Activating Proteins
  • rab5 GTP-Binding Proteins
  • rab GTP-Binding Proteins
  • Virus Internalization
  • Tight Junctions
  • Occludin
  • Membrane Proteins
  • Immunology
  • Humans
  • Epithelial Cells