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A guide to interrogating immunometabolism.

Publication ,  Journal Article
Voss, K; Hong, HS; Bader, JE; Sugiura, A; Lyssiotis, CA; Rathmell, JC
Published in: Nature reviews. Immunology
October 2021

The metabolic charts memorized in early biochemistry courses, and then later forgotten, have come back to haunt many immunologists with new recognition of the importance of these pathways. Metabolites and the activity of metabolic pathways drive energy production, macromolecule synthesis, intracellular signalling, post-translational modifications and cell survival. Immunologists who identify a metabolic phenotype in their system are often left wondering where to begin and what does it mean? Here, we provide a framework for navigating and selecting the appropriate biochemical techniques to explore immunometabolism. We offer recommendations for initial approaches to develop and test metabolic hypotheses and how to avoid common mistakes. We then discuss how to take things to the next level with metabolomic approaches, such as isotope tracing and genetic approaches. By proposing strategies and evaluating the strengths and weaknesses of different methodologies, we aim to provide insight, note important considerations and discuss ways to avoid common misconceptions. Furthermore, we highlight recent studies demonstrating the power of these metabolic approaches to uncover the role of metabolism in immunology. By following the framework in this Review, neophytes and seasoned investigators alike can venture into the emerging realm of cellular metabolism and immunity with confidence and rigour.

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Published In

Nature reviews. Immunology

DOI

EISSN

1474-1741

ISSN

1474-1733

Publication Date

October 2021

Volume

21

Issue

10

Start / End Page

637 / 652

Related Subject Headings

  • Mitochondria
  • Metabolomics
  • Metabolic Networks and Pathways
  • Immunology
  • Immunity
  • Humans
  • Glycolysis
  • Animals
  • 3204 Immunology
  • 1107 Immunology
 

Citation

APA
Chicago
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Voss, K., Hong, H. S., Bader, J. E., Sugiura, A., Lyssiotis, C. A., & Rathmell, J. C. (2021). A guide to interrogating immunometabolism. Nature Reviews. Immunology, 21(10), 637–652. https://doi.org/10.1038/s41577-021-00529-8
Voss, Kelsey, Hanna S. Hong, Jackie E. Bader, Ayaka Sugiura, Costas A. Lyssiotis, and Jeffrey C. Rathmell. “A guide to interrogating immunometabolism.Nature Reviews. Immunology 21, no. 10 (October 2021): 637–52. https://doi.org/10.1038/s41577-021-00529-8.
Voss K, Hong HS, Bader JE, Sugiura A, Lyssiotis CA, Rathmell JC. A guide to interrogating immunometabolism. Nature reviews Immunology. 2021 Oct;21(10):637–52.
Voss, Kelsey, et al. “A guide to interrogating immunometabolism.Nature Reviews. Immunology, vol. 21, no. 10, Oct. 2021, pp. 637–52. Epmc, doi:10.1038/s41577-021-00529-8.
Voss K, Hong HS, Bader JE, Sugiura A, Lyssiotis CA, Rathmell JC. A guide to interrogating immunometabolism. Nature reviews Immunology. 2021 Oct;21(10):637–652.

Published In

Nature reviews. Immunology

DOI

EISSN

1474-1741

ISSN

1474-1733

Publication Date

October 2021

Volume

21

Issue

10

Start / End Page

637 / 652

Related Subject Headings

  • Mitochondria
  • Metabolomics
  • Metabolic Networks and Pathways
  • Immunology
  • Immunity
  • Humans
  • Glycolysis
  • Animals
  • 3204 Immunology
  • 1107 Immunology