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When next-generation sequencing-based preimplantation genetic testing for aneuploidy (PGT-A) yields an inconclusive report: diagnostic results and clinical outcomes after re biopsy.

Publication ,  Journal Article
Neal, SA; Sun, L; Jalas, C; Morin, SJ; Molinaro, TA; Scott, RT
Published in: J Assist Reprod Genet
October 2019

PURPOSE: To describe diagnostic results following re-biopsy of blastocysts with inconclusive results on preimplantation genetic screening for aneuploidy (PGT-A) and to evaluate the reproductive potential of re-biopsied blastocysts. METHODS: This retrospective cohort study included all trophectoderm biopsies submitted for PGT-A by a large in vitro fertilization center to a single genetics laboratory from June 2016 to October 2018. PGT-A was performed using next-generation sequencing (NGS). No-result blastocysts that underwent re-biopsy were subsequently classified as euploid, aneuploid, mosaic/segmental, or no-result. Ongoing pregnancy and clinical loss rates were assessed following transfer of re-biopsied blastocysts. Logistic regressions were conducted to account for age and blastocyst morphology. RESULTS: Of the trophectoderm biopsies submitted for PGT-A, 635/25,199 (2.5%) were categorized as no-result. Those that underwent re-biopsy (n = 250) had a 95.2% diagnostic rate with 140 (56.0%) receiving euploid diagnoses. Thirty-six re-biopsied blastocysts deemed euploid were subsequently transferred, resulting in 18 (50.0%) ongoing pregnancies and 5 (13.9%) clinical losses. After adjusting for age and blastocyst morphology, there remained a lower ongoing pregnancy rate and a trend towards higher clinical loss rate following transfer of a re-biopsied blastocyst. When compared to blastocysts that underwent the same number of vitrification-warming cycles but only one biopsy, there were no differences in outcomes. CONCLUSIONS: Failure to obtain an analytical result does not change the probability that a given blastocyst is euploid. Pregnancy outcomes following transfer of re-biopsied blastocysts are favorable, but further data must be accrued for an adequately powered comparison with outcomes after transfer of blastocysts biopsied once.

Duke Scholars

Published In

J Assist Reprod Genet

DOI

EISSN

1573-7330

Publication Date

October 2019

Volume

36

Issue

10

Start / End Page

2103 / 2109

Location

Netherlands

Related Subject Headings

  • Preimplantation Diagnosis
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Logistic Models
  • Humans
  • High-Throughput Nucleotide Sequencing
  • Female
  • Ectoderm
  • Blastocyst
  • Biopsy
 

Citation

APA
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MLA
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Neal, S. A., Sun, L., Jalas, C., Morin, S. J., Molinaro, T. A., & Scott, R. T. (2019). When next-generation sequencing-based preimplantation genetic testing for aneuploidy (PGT-A) yields an inconclusive report: diagnostic results and clinical outcomes after re biopsy. J Assist Reprod Genet, 36(10), 2103–2109. https://doi.org/10.1007/s10815-019-01550-6
Neal, Shelby A., L. Sun, C. Jalas, S. J. Morin, T. A. Molinaro, and R. T. Scott. “When next-generation sequencing-based preimplantation genetic testing for aneuploidy (PGT-A) yields an inconclusive report: diagnostic results and clinical outcomes after re biopsy.J Assist Reprod Genet 36, no. 10 (October 2019): 2103–9. https://doi.org/10.1007/s10815-019-01550-6.
Neal, Shelby A., et al. “When next-generation sequencing-based preimplantation genetic testing for aneuploidy (PGT-A) yields an inconclusive report: diagnostic results and clinical outcomes after re biopsy.J Assist Reprod Genet, vol. 36, no. 10, Oct. 2019, pp. 2103–09. Pubmed, doi:10.1007/s10815-019-01550-6.
Journal cover image

Published In

J Assist Reprod Genet

DOI

EISSN

1573-7330

Publication Date

October 2019

Volume

36

Issue

10

Start / End Page

2103 / 2109

Location

Netherlands

Related Subject Headings

  • Preimplantation Diagnosis
  • Pregnancy
  • Obstetrics & Reproductive Medicine
  • Logistic Models
  • Humans
  • High-Throughput Nucleotide Sequencing
  • Female
  • Ectoderm
  • Blastocyst
  • Biopsy