Using BioID to Characterize the RAS Interactome.
Publication
, Journal Article
Adhikari, H; Counter, CM
Published in: Methods Mol Biol
2021
Identifying the proteins that associate with RAS oncoproteins has great potential, not only to elucidate how these mutant proteins are regulated and signal but also to identify potential therapeutic targets. Here we describe a detailed protocol to employ proximity labeling by the BioID methodology, which has the advantage of capturing weak or transient interactions, to identify in an unbiased manner those proteins within the immediate vicinity of oncogenic RAS proteins.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2021
Volume
2262
Start / End Page
271 / 280
Location
United States
Related Subject Headings
- ras Proteins
- Protein Interaction Mapping
- Protein Interaction Domains and Motifs
- Protein Binding
- Humans
- Developmental Biology
- Biotinylation
- Biotin
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Adhikari, H., & Counter, C. M. (2021). Using BioID to Characterize the RAS Interactome. Methods Mol Biol, 2262, 271–280. https://doi.org/10.1007/978-1-0716-1190-6_16
Adhikari, Hema, and Christopher M. Counter. “Using BioID to Characterize the RAS Interactome.” Methods Mol Biol 2262 (2021): 271–80. https://doi.org/10.1007/978-1-0716-1190-6_16.
Adhikari H, Counter CM. Using BioID to Characterize the RAS Interactome. Methods Mol Biol. 2021;2262:271–80.
Adhikari, Hema, and Christopher M. Counter. “Using BioID to Characterize the RAS Interactome.” Methods Mol Biol, vol. 2262, 2021, pp. 271–80. Pubmed, doi:10.1007/978-1-0716-1190-6_16.
Adhikari H, Counter CM. Using BioID to Characterize the RAS Interactome. Methods Mol Biol. 2021;2262:271–280.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2021
Volume
2262
Start / End Page
271 / 280
Location
United States
Related Subject Headings
- ras Proteins
- Protein Interaction Mapping
- Protein Interaction Domains and Motifs
- Protein Binding
- Humans
- Developmental Biology
- Biotinylation
- Biotin
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology