Skip to main content

Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing.

Publication ,  Journal Article
Koldaeva, A; Zhang, C; Huang, Y-P; Reinert, JK; Mizuno, S; Sugiyama, F; Takahashi, S; Soliman, T; Matsunami, H; Fukunaga, I
Published in: J Neurosci
July 28, 2021

In sensory systems of the brain, mechanisms exist to extract distinct features from stimuli to generate a variety of behavioral repertoires. These often correspond to different cell types at various stages in sensory processing. In the mammalian olfactory system, complex information processing starts in the olfactory bulb, whose output is conveyed by mitral cells (MCs) and tufted cells (TCs). Despite many differences between them, and despite the crucial position they occupy in the information hierarchy, Cre-driver lines that distinguish them do not yet exist. Here, we sought to identify genes that are differentially expressed between MCs and TCs of the mouse, with an ultimate goal to generate a cell type-specific Cre-driver line, starting from a transcriptome analysis using a large and publicly available single-cell RNA-seq dataset (Zeisel et al., 2018). Many genes were differentially expressed, but only a few showed consistent expressions in MCs and at the specificity required. After further validating these putative markers using ISH, two genes (i.e., Pkib and Lbdh2) remained as promising candidates. Using CRISPR/Cas9-mediated gene editing, we generated Cre-driver lines and analyzed the resulting recombination patterns. This indicated that our new inducible Cre-driver line, Lbhd2-CreERT2, can be used to genetically label MCs in a tamoxifen dose-dependent manner, both in male and female mice, as assessed by soma locations, projection patterns, and sensory-evoked responses in vivo Hence, this is a promising tool for investigating cell type-specific contributions to olfactory processing and demonstrates the power of publicly accessible data in accelerating science.SIGNIFICANCE STATEMENT In the brain, distinct cell types play unique roles. It is therefore important to have tools for studying unique cell types specifically. For the sense of smell in mammals, information is processed first by circuits of the olfactory bulb, where two types of cells, mitral cells and tufted cells, output different information. We generated a transgenic mouse line that enables mitral cells to be specifically labeled or manipulated. This was achieved by looking for genes that are specific to mitral cells using a large and public gene expression dataset, and creating a transgenic mouse using the gene editing technique, CRISPR/Cas9. This will allow scientists to better investigate parallel information processing underlying the sense of smell.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

July 28, 2021

Volume

41

Issue

30

Start / End Page

6449 / 6467

Location

United States

Related Subject Headings

  • Olfactory Perception
  • Olfactory Pathways
  • Olfactory Bulb
  • Neurons
  • Neurology & Neurosurgery
  • Mice, Transgenic
  • Mice
  • Male
  • Integrases
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Koldaeva, A., Zhang, C., Huang, Y.-P., Reinert, J. K., Mizuno, S., Sugiyama, F., … Fukunaga, I. (2021). Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing. J Neurosci, 41(30), 6449–6467. https://doi.org/10.1523/JNEUROSCI.3076-20.2021
Koldaeva, Anzhelika, Cary Zhang, Yu-Pei Huang, Janine Kristin Reinert, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Taha Soliman, Hiroaki Matsunami, and Izumi Fukunaga. “Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing.J Neurosci 41, no. 30 (July 28, 2021): 6449–67. https://doi.org/10.1523/JNEUROSCI.3076-20.2021.
Koldaeva A, Zhang C, Huang Y-P, Reinert JK, Mizuno S, Sugiyama F, et al. Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing. J Neurosci. 2021 Jul 28;41(30):6449–67.
Koldaeva, Anzhelika, et al. “Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing.J Neurosci, vol. 41, no. 30, July 2021, pp. 6449–67. Pubmed, doi:10.1523/JNEUROSCI.3076-20.2021.
Koldaeva A, Zhang C, Huang Y-P, Reinert JK, Mizuno S, Sugiyama F, Takahashi S, Soliman T, Matsunami H, Fukunaga I. Generation and Characterization of a Cell Type-Specific, Inducible Cre-Driver Line to Study Olfactory Processing. J Neurosci. 2021 Jul 28;41(30):6449–6467.

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

July 28, 2021

Volume

41

Issue

30

Start / End Page

6449 / 6467

Location

United States

Related Subject Headings

  • Olfactory Perception
  • Olfactory Pathways
  • Olfactory Bulb
  • Neurons
  • Neurology & Neurosurgery
  • Mice, Transgenic
  • Mice
  • Male
  • Integrases
  • Female