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Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract.

Publication ,  Journal Article
Manickam, C; Nwanze, C; Ram, DR; Shah, SV; Smith, S; Jones, R; Hueber, B; Kroll, K; Varner, V; Goepfert, P; Jost, S; Reeves, RK
Published in: AIDS
July 31, 2018

OBJECTIVE: Recently, a seemingly novel innate immune cell subset bearing features of natural killer and B cells was identified in mice. So-called NKB cells appear as first responders to infections, but whether this cell population is truly novel or is in fact a subpopulation of B cells and exists in higher primates remains unclear. The objective of this study was to identify NKB cells in primates and study the impact of HIV/SIV infections. DESIGN AND METHODS: NKB cells were quantified in both naive and lentivirus infected rhesus macaques and humans by excluding lineage markers (CD3, CD127) and positive Boolean gating for CD20, NKG2A/C and/or NKp46. Additional phenotypic measures were conducted by RNA-probe and traditional flow cytometry. RESULTS: Circulating cytotoxic NKB cells were found at similar frequencies in humans and rhesus macaques (range, 0.01-0.2% of total lymphocytes). NKB cells were notably enriched in spleen (median, 0.4% of lymphocytes), but were otherwise systemically distributed in tonsil, lymph nodes, colon, and jejunum. Expression of immunoglobulin was highly variable, but heavily favoured IgM and IgA rather than IgG. Interestingly, NKB cell frequencies expanded in PBMC and colon during SIV infection, as did IgG expression, but were generally unaltered in HIV-infected humans. CONCLUSION: These results suggest a cell type expressing both natural killer and B-cell features exists in rhesus macaques and humans and are perturbed by HIV/SIV infection. The full functional niche remains unknown, but the unique phenotype and systemic distribution could make NKB cells unique targets for immunotherapeutics or vaccine strategies.

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Published In

AIDS

DOI

EISSN

1473-5571

Publication Date

July 31, 2018

Volume

32

Issue

12

Start / End Page

1571 / 1578

Location

England

Related Subject Headings

  • Virology
  • Simian Acquired Immunodeficiency Syndrome
  • Receptors, Natural Killer Cell
  • Middle Aged
  • Macaca mulatta
  • Lymphocyte Count
  • Immunoglobulin M
  • Immunoglobulin A
  • Humans
  • HIV Infections
 

Citation

APA
Chicago
ICMJE
MLA
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Manickam, C., Nwanze, C., Ram, D. R., Shah, S. V., Smith, S., Jones, R., … Reeves, R. K. (2018). Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract. AIDS, 32(12), 1571–1578. https://doi.org/10.1097/QAD.0000000000001855
Manickam, Cordelia, Chiadika Nwanze, Daniel R. Ram, Spandan V. Shah, Scott Smith, Rhianna Jones, Brady Hueber, et al. “Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract.AIDS 32, no. 12 (July 31, 2018): 1571–78. https://doi.org/10.1097/QAD.0000000000001855.
Manickam C, Nwanze C, Ram DR, Shah SV, Smith S, Jones R, et al. Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract. AIDS. 2018 Jul 31;32(12):1571–8.
Manickam, Cordelia, et al. “Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract.AIDS, vol. 32, no. 12, July 2018, pp. 1571–78. Pubmed, doi:10.1097/QAD.0000000000001855.
Manickam C, Nwanze C, Ram DR, Shah SV, Smith S, Jones R, Hueber B, Kroll K, Varner V, Goepfert P, Jost S, Reeves RK. Progressive lentivirus infection induces natural killer cell receptor-expressing B cells in the gastrointestinal tract. AIDS. 2018 Jul 31;32(12):1571–1578.

Published In

AIDS

DOI

EISSN

1473-5571

Publication Date

July 31, 2018

Volume

32

Issue

12

Start / End Page

1571 / 1578

Location

England

Related Subject Headings

  • Virology
  • Simian Acquired Immunodeficiency Syndrome
  • Receptors, Natural Killer Cell
  • Middle Aged
  • Macaca mulatta
  • Lymphocyte Count
  • Immunoglobulin M
  • Immunoglobulin A
  • Humans
  • HIV Infections