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The use of tocofersolan as a rescue agent in larval zebrafish exposed to benzo[a]pyrene in early development.

Publication ,  Journal Article
Holloway, Z; Hawkey, A; Asrat, H; Boinapally, N; Levin, ED
Published in: Neurotoxicology
September 2021

Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental pollutants created by incomplete combustion. Benzo(a)pyrene (BaP), the prototypic PAH, is known to exert toxicity through oxidative stress which is thought to occur through inhibition of antioxidant scavenging systems. The use of agents that reduce oxidative stress may be a valuable route for ameliorating the adverse effects of PAHs on neural development and behavior. This study was conducted to determine if tocofersolan (a synthetic water-soluble analog of vitamin E) supplementation can prevent or reduce neurobehavioral deficits in zebrafish embryos exposed to BaP during early development. Newly hatched zebrafish were assessed on locomotor activity and light responsivity. Zebrafish embryos were exposed to vehicle (DMSO), tocofersolan (0.3 μM-3 μM), and/or BaP (5 μM) from 5-120 hours post-fertilization. This concentration range was below the threshold for producing overt dysmorphogenesis or decreased survival. One day after the end of exposure the larval fish were tested for locomotor activity under alternating light and dark 10 min periods, BaP (5 μM) was found to cause locomotor hypoactivity in larval fish. Co-exposure of tocofersolan (1 μM) restored control-like locomotor function. Based on the findings of this study, this model can be expanded to assess the outcome of vitamin E supplementation on other potential environmental neurotoxicants, and lead to determination if this rescue persists into adulthood.

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Published In

Neurotoxicology

DOI

EISSN

1872-9711

Publication Date

September 2021

Volume

86

Start / End Page

78 / 84

Location

Netherlands

Related Subject Headings

  • Zebrafish
  • Water Pollutants, Chemical
  • Vitamin E
  • Toxicology
  • Oxidative Stress
  • Male
  • Locomotion
  • Larva
  • Female
  • Benzo(a)pyrene
 

Citation

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Holloway, Z., Hawkey, A., Asrat, H., Boinapally, N., & Levin, E. D. (2021). The use of tocofersolan as a rescue agent in larval zebrafish exposed to benzo[a]pyrene in early development. Neurotoxicology, 86, 78–84. https://doi.org/10.1016/j.neuro.2021.07.003
Holloway, Zade, Andrew Hawkey, Helina Asrat, Nidhi Boinapally, and Edward D. Levin. “The use of tocofersolan as a rescue agent in larval zebrafish exposed to benzo[a]pyrene in early development.Neurotoxicology 86 (September 2021): 78–84. https://doi.org/10.1016/j.neuro.2021.07.003.
Holloway Z, Hawkey A, Asrat H, Boinapally N, Levin ED. The use of tocofersolan as a rescue agent in larval zebrafish exposed to benzo[a]pyrene in early development. Neurotoxicology. 2021 Sep;86:78–84.
Holloway, Zade, et al. “The use of tocofersolan as a rescue agent in larval zebrafish exposed to benzo[a]pyrene in early development.Neurotoxicology, vol. 86, Sept. 2021, pp. 78–84. Pubmed, doi:10.1016/j.neuro.2021.07.003.
Holloway Z, Hawkey A, Asrat H, Boinapally N, Levin ED. The use of tocofersolan as a rescue agent in larval zebrafish exposed to benzo[a]pyrene in early development. Neurotoxicology. 2021 Sep;86:78–84.
Journal cover image

Published In

Neurotoxicology

DOI

EISSN

1872-9711

Publication Date

September 2021

Volume

86

Start / End Page

78 / 84

Location

Netherlands

Related Subject Headings

  • Zebrafish
  • Water Pollutants, Chemical
  • Vitamin E
  • Toxicology
  • Oxidative Stress
  • Male
  • Locomotion
  • Larva
  • Female
  • Benzo(a)pyrene