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Evaluation of Antimicrobial Susceptibility Testing Methods for Burkholderia cenocepacia and Burkholderia multivorans Isolates from Cystic Fibrosis Patients.

Publication ,  Journal Article
Huse, HK; Lee, MJ; Wootton, M; Sharp, SE; Traczewski, M; LiPuma, JJ; Jorth, P
Published in: J Clin Microbiol
November 18, 2021

The Burkholderia cepacia complex (BCC) is known for causing serious lung infections in people with cystic fibrosis (CF). These infections can require lung transplantation, eligibility for which may be guided by antimicrobial susceptibility testing (AST). While the Clinical and Laboratory Standards Institute recommends AST for BCC, the European Committee on Antimicrobial Susceptibility Testing (EUCAST) does not, due to poor method performance and correlation with clinical outcomes. Furthermore, limited data exist on the performance of automated AST methods for BCC. To address these issues, reproducibility and accuracy were evaluated for disk diffusion (DD), broth microdilution (BMD), and MicroScan WalkAway using 50 B. cenocepacia and 50 B. multivorans isolates collected from people with CF. The following drugs were evaluated in triplicate: chloramphenicol (CAM), ceftazidime (CAZ), meropenem (MEM), trimethoprim-sulfamethoxazole (TMP-SMX), minocycline (MIN), levofloxacin (LVX), ciprofloxacin (CIP), and piperacillin-tazobactam (PIP-TAZ). BMD reproducibility was ≥ 95% for MEM and MIN only, and MicroScan WalkAway reproducibility was similar to BMD. DD reproducibility was < 90% for all drugs tested when a 3 mm cut-off was applied. When comparing the accuracy of DD to BMD, only MEM met all acceptance criteria. TMP-SMX and LVX had high minor errors, CAZ had unacceptable very major errors (VME), and MIN, PIP-TAZ, and CIP had both unacceptable minor errors and VMEs. For MicroScan WalkAway, no drugs met acceptance criteria. Analyses also showed that errors were not attributed to one species. In general, our data agree with EUCAST recommendations.

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Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

November 18, 2021

Volume

59

Issue

12

Start / End Page

e0144721

Location

United States

Related Subject Headings

  • Reproducibility of Results
  • Microbiology
  • Microbial Sensitivity Tests
  • Humans
  • Cystic Fibrosis
  • Burkholderia cepacia complex
  • Burkholderia cenocepacia
  • Burkholderia Infections
  • Burkholderia
  • Anti-Bacterial Agents
 

Citation

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Huse, H. K., Lee, M. J., Wootton, M., Sharp, S. E., Traczewski, M., LiPuma, J. J., & Jorth, P. (2021). Evaluation of Antimicrobial Susceptibility Testing Methods for Burkholderia cenocepacia and Burkholderia multivorans Isolates from Cystic Fibrosis Patients. J Clin Microbiol, 59(12), e0144721. https://doi.org/10.1128/JCM.01447-21
Huse, Holly K., Mark J. Lee, Mandy Wootton, Susan E. Sharp, Maria Traczewski, John J. LiPuma, and Peter Jorth. “Evaluation of Antimicrobial Susceptibility Testing Methods for Burkholderia cenocepacia and Burkholderia multivorans Isolates from Cystic Fibrosis Patients.J Clin Microbiol 59, no. 12 (November 18, 2021): e0144721. https://doi.org/10.1128/JCM.01447-21.
Huse HK, Lee MJ, Wootton M, Sharp SE, Traczewski M, LiPuma JJ, et al. Evaluation of Antimicrobial Susceptibility Testing Methods for Burkholderia cenocepacia and Burkholderia multivorans Isolates from Cystic Fibrosis Patients. J Clin Microbiol. 2021 Nov 18;59(12):e0144721.
Huse, Holly K., et al. “Evaluation of Antimicrobial Susceptibility Testing Methods for Burkholderia cenocepacia and Burkholderia multivorans Isolates from Cystic Fibrosis Patients.J Clin Microbiol, vol. 59, no. 12, Nov. 2021, p. e0144721. Pubmed, doi:10.1128/JCM.01447-21.
Huse HK, Lee MJ, Wootton M, Sharp SE, Traczewski M, LiPuma JJ, Jorth P. Evaluation of Antimicrobial Susceptibility Testing Methods for Burkholderia cenocepacia and Burkholderia multivorans Isolates from Cystic Fibrosis Patients. J Clin Microbiol. 2021 Nov 18;59(12):e0144721.

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

November 18, 2021

Volume

59

Issue

12

Start / End Page

e0144721

Location

United States

Related Subject Headings

  • Reproducibility of Results
  • Microbiology
  • Microbial Sensitivity Tests
  • Humans
  • Cystic Fibrosis
  • Burkholderia cepacia complex
  • Burkholderia cenocepacia
  • Burkholderia Infections
  • Burkholderia
  • Anti-Bacterial Agents