Detection of necrosis by release of lactate dehydrogenase activity.
Publication
, Journal Article
Chan, FK-M; Moriwaki, K; De Rosa, MJ
Published in: Methods Mol Biol
2013
Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2013
Volume
979
Start / End Page
65 / 70
Location
United States
Related Subject Headings
- Tetrazolium Salts
- Necrosis
- NAD
- L-Lactate Dehydrogenase
- Humans
- Electron Transport
- Developmental Biology
- Colorimetry
- Cell Culture Techniques
- Biocatalysis
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MLA
NLM
Chan, F.-M., Moriwaki, K., & De Rosa, M. J. (2013). Detection of necrosis by release of lactate dehydrogenase activity. Methods Mol Biol, 979, 65–70. https://doi.org/10.1007/978-1-62703-290-2_7
Chan, Francis Ka-Ming, Kenta Moriwaki, and María José De Rosa. “Detection of necrosis by release of lactate dehydrogenase activity.” Methods Mol Biol 979 (2013): 65–70. https://doi.org/10.1007/978-1-62703-290-2_7.
Chan FK-M, Moriwaki K, De Rosa MJ. Detection of necrosis by release of lactate dehydrogenase activity. Methods Mol Biol. 2013;979:65–70.
Chan, Francis Ka-Ming, et al. “Detection of necrosis by release of lactate dehydrogenase activity.” Methods Mol Biol, vol. 979, 2013, pp. 65–70. Pubmed, doi:10.1007/978-1-62703-290-2_7.
Chan FK-M, Moriwaki K, De Rosa MJ. Detection of necrosis by release of lactate dehydrogenase activity. Methods Mol Biol. 2013;979:65–70.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2013
Volume
979
Start / End Page
65 / 70
Location
United States
Related Subject Headings
- Tetrazolium Salts
- Necrosis
- NAD
- L-Lactate Dehydrogenase
- Humans
- Electron Transport
- Developmental Biology
- Colorimetry
- Cell Culture Techniques
- Biocatalysis