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Enterocyte-innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium.

Publication ,  Journal Article
Gullicksrud, JA; Sateriale, A; Engiles, JB; Gibson, AR; Shaw, S; Hutchins, ZA; Martin, L; Christian, DA; Taylor, GA; Yamamoto, M; Beiting, DP ...
Published in: Mucosal Immunol
February 2022

The intestinal parasite, Cryptosporidium, is a major contributor to global child mortality and causes opportunistic infection in immune deficient individuals. Innate resistance to Cryptosporidium, which specifically invades enterocytes, is dependent on the production of IFN-γ, yet whether enterocytes contribute to parasite control is poorly understood. In this study, utilizing a mouse-adapted strain of C. parvum, we show that epithelial-derived IL-18 synergized with IL-12 to stimulate innate lymphoid cell (ILC) production of IFN-γ required for early parasite control. The loss of IFN-γ-mediated STAT1 signaling in enterocytes, but not dendritic cells or macrophages, antagonized early parasite control. Transcriptional profiling of enterocytes from infected mice identified an IFN-γ signature and enrichment of the anti-microbial effectors IDO, GBP, and IRG. Deletion experiments identified a role for Irgm1/m3 in parasite control. Thus, enterocytes promote ILC production of IFN-γ that acts on enterocytes to restrict the growth of Cryptosporidium.

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Published In

Mucosal Immunol

DOI

EISSN

1935-3456

Publication Date

February 2022

Volume

15

Issue

2

Start / End Page

362 / 372

Location

United States

Related Subject Headings

  • Mice
  • Lymphocytes
  • Immunology
  • Immunity, Innate
  • Humans
  • Enterocytes
  • Cryptosporidium parvum
  • Cryptosporidium
  • Cryptosporidiosis
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
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Gullicksrud, J. A., Sateriale, A., Engiles, J. B., Gibson, A. R., Shaw, S., Hutchins, Z. A., … Hunter, C. A. (2022). Enterocyte-innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium. Mucosal Immunol, 15(2), 362–372. https://doi.org/10.1038/s41385-021-00468-6
Gullicksrud, Jodi A., Adam Sateriale, Julie B. Engiles, Alexis R. Gibson, Sebastian Shaw, Zachary A. Hutchins, Lindsay Martin, et al. “Enterocyte-innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium.Mucosal Immunol 15, no. 2 (February 2022): 362–72. https://doi.org/10.1038/s41385-021-00468-6.
Gullicksrud JA, Sateriale A, Engiles JB, Gibson AR, Shaw S, Hutchins ZA, et al. Enterocyte-innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium. Mucosal Immunol. 2022 Feb;15(2):362–72.
Gullicksrud, Jodi A., et al. “Enterocyte-innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium.Mucosal Immunol, vol. 15, no. 2, Feb. 2022, pp. 362–72. Pubmed, doi:10.1038/s41385-021-00468-6.
Gullicksrud JA, Sateriale A, Engiles JB, Gibson AR, Shaw S, Hutchins ZA, Martin L, Christian DA, Taylor GA, Yamamoto M, Beiting DP, Striepen B, Hunter CA. Enterocyte-innate lymphoid cell crosstalk drives early IFN-γ-mediated control of Cryptosporidium. Mucosal Immunol. 2022 Feb;15(2):362–372.

Published In

Mucosal Immunol

DOI

EISSN

1935-3456

Publication Date

February 2022

Volume

15

Issue

2

Start / End Page

362 / 372

Location

United States

Related Subject Headings

  • Mice
  • Lymphocytes
  • Immunology
  • Immunity, Innate
  • Humans
  • Enterocytes
  • Cryptosporidium parvum
  • Cryptosporidium
  • Cryptosporidiosis
  • Animals