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Proton-gated ion channels in mouse bone marrow stromal cells.

Publication ,  Journal Article
Swain, SM; Parameswaran, S; Sahu, G; Verma, RS; Bera, AK
Published in: Stem Cell Res
September 2012

A variety of ion channels like acid sensing ion channels (ASICs) and several members of the transient receptor potential (TRP) cation channel family are known to be activated by protons. The present study describes proton-gated current in mouse bone marrow stromal cells (BMSCs), by using whole cell patch clamp. Rapid application of extracellular solution of pH ≤ 6.5, evoked slow inactivating current with mean peak value of 328 ± 31pA, (n = 25) at pH 5.0. The reversal potential was close to the theoretical Na(+) equilibrium potential, indicating that majority of the current is mediated by Na(+) and partially carried by Ca(2+) as revealed by ion substitution experiments and Ca(2+) imaging. ASICs blocker amiloride (1mM) and nonselective cation channel blocker flufenamic acid (0.3mM) reduced the current amplitudes by 36 ± 5% (n = 10) and 39 ± 7% (n = 14) respectively. Co-application of flufenamic acid and amiloride further decreased the current by 70 ± 7% (n = 7). However, capsazepine, SKF 96365 and ruthenium red had no effect. 10mM of Ca(2+) and 2mM of La(3+) inhibited the current by 39 ± 6% (n = 5) and 46 ± 6% (n = 4) respectively. Zn(2+) (300 μM) and Gd(3+) (500 μM) had no effect on the current amplitude. Low pH mediated cell death was completely inhibited by co-application of La(3+) and amiloride. Reverse Transcriptase-PCR detected expression of mRNAs of ASICs and TRP family. In summary, our results demonstrate the functional expression of low pH-activated ion channels in mouse BMSCs.

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Published In

Stem Cell Res

DOI

EISSN

1876-7753

Publication Date

September 2012

Volume

9

Issue

2

Start / End Page

59 / 68

Location

England

Related Subject Headings

  • Transient Receptor Potential Channels
  • Sodium
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Protons
  • Mice
  • Mesenchymal Stem Cells
  • Ions
  • Ion Channel Gating
  • Hydrogen-Ion Concentration
 

Citation

APA
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Swain, S. M., Parameswaran, S., Sahu, G., Verma, R. S., & Bera, A. K. (2012). Proton-gated ion channels in mouse bone marrow stromal cells. Stem Cell Res, 9(2), 59–68. https://doi.org/10.1016/j.scr.2012.04.005
Swain, Sandip Madhusudan, Sreejit Parameswaran, Giriraj Sahu, Rama Shanker Verma, and Amal Kanti Bera. “Proton-gated ion channels in mouse bone marrow stromal cells.Stem Cell Res 9, no. 2 (September 2012): 59–68. https://doi.org/10.1016/j.scr.2012.04.005.
Swain SM, Parameswaran S, Sahu G, Verma RS, Bera AK. Proton-gated ion channels in mouse bone marrow stromal cells. Stem Cell Res. 2012 Sep;9(2):59–68.
Swain, Sandip Madhusudan, et al. “Proton-gated ion channels in mouse bone marrow stromal cells.Stem Cell Res, vol. 9, no. 2, Sept. 2012, pp. 59–68. Pubmed, doi:10.1016/j.scr.2012.04.005.
Swain SM, Parameswaran S, Sahu G, Verma RS, Bera AK. Proton-gated ion channels in mouse bone marrow stromal cells. Stem Cell Res. 2012 Sep;9(2):59–68.

Published In

Stem Cell Res

DOI

EISSN

1876-7753

Publication Date

September 2012

Volume

9

Issue

2

Start / End Page

59 / 68

Location

England

Related Subject Headings

  • Transient Receptor Potential Channels
  • Sodium
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger
  • Protons
  • Mice
  • Mesenchymal Stem Cells
  • Ions
  • Ion Channel Gating
  • Hydrogen-Ion Concentration