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Macrophage imaging within human cerebral aneurysms wall using ferumoxytol-enhanced MRI: a pilot study.

Publication ,  Journal Article
Hasan, DM; Mahaney, KB; Magnotta, VA; Kung, DK; Lawton, MT; Hashimoto, T; Winn, HR; Saloner, D; Martin, A; Gahramanov, S; Dósa, E; Neuwelt, E ...
Published in: Arterioscler Thromb Vasc Biol
April 2012

OBJECTIVE: Macrophages play a critical role in cerebral aneurysm formation and rupture. The purpose of this study is to demonstrate the feasibility and optimal parameters of imaging macrophages within human cerebral aneurysm wall using ferumoxytol-enhanced MRI. METHODS AND RESULTS: Nineteen unruptured aneurysms in 11 patients were imaged using T2*-GE-MRI sequence. Two protocols were used. Protocol A was an infusion of 2.5 mg/kg of ferumoxytol and imaging at day 0 and 1. Protocol B was an infusion of 5 mg/kg of ferumoxytol and imaging at day 0 and 3. All images were reviewed independently by 2 neuroradiologists to assess for ferumoxytol-associated loss of MRI signal intensity within aneurysm wall. Aneurysm tissue was harvested for histological analysis. Fifty percent (5/10) of aneurysms in protocol A showed ferumoxytol-associated signal changes in aneurysm walls compared to 78% (7/9) of aneurysms in protocol B. Aneurysm tissue harvested from patients infused with ferumoxytol stained positive for both CD68+, demonstrating macrophage infiltration, and Prussian blue, demonstrating uptake of iron particles. Tissue harvested from controls stained positive for CD68 but not Prussian blue. CONCLUSIONS: Imaging with T2*-GE-MRI at 72 hours postinfusion of 5 mg/kg of ferumoxytol establishes a valid and useful approximation of optimal dose and timing parameters for macrophages imaging within aneurysm wall. Further studies are needed to correlate these imaging findings with risk of intracranial aneurysm rupture.

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Published In

Arterioscler Thromb Vasc Biol

DOI

EISSN

1524-4636

Publication Date

April 2012

Volume

32

Issue

4

Start / End Page

1032 / 1038

Location

United States

Related Subject Headings

  • Time Factors
  • Staining and Labeling
  • Reproducibility of Results
  • Prospective Studies
  • Predictive Value of Tests
  • Pilot Projects
  • Middle Aged
  • Male
  • Magnetic Resonance Imaging
  • Macrophages
 

Citation

APA
Chicago
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MLA
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Hasan, D. M., Mahaney, K. B., Magnotta, V. A., Kung, D. K., Lawton, M. T., Hashimoto, T., … Young, W. L. (2012). Macrophage imaging within human cerebral aneurysms wall using ferumoxytol-enhanced MRI: a pilot study. Arterioscler Thromb Vasc Biol, 32(4), 1032–1038. https://doi.org/10.1161/ATVBAHA.111.239871
Hasan, David M., Kelly B. Mahaney, Vincent A. Magnotta, David K. Kung, Michael T. Lawton, Tomoki Hashimoto, H Richard Winn, et al. “Macrophage imaging within human cerebral aneurysms wall using ferumoxytol-enhanced MRI: a pilot study.Arterioscler Thromb Vasc Biol 32, no. 4 (April 2012): 1032–38. https://doi.org/10.1161/ATVBAHA.111.239871.
Hasan DM, Mahaney KB, Magnotta VA, Kung DK, Lawton MT, Hashimoto T, et al. Macrophage imaging within human cerebral aneurysms wall using ferumoxytol-enhanced MRI: a pilot study. Arterioscler Thromb Vasc Biol. 2012 Apr;32(4):1032–8.
Hasan, David M., et al. “Macrophage imaging within human cerebral aneurysms wall using ferumoxytol-enhanced MRI: a pilot study.Arterioscler Thromb Vasc Biol, vol. 32, no. 4, Apr. 2012, pp. 1032–38. Pubmed, doi:10.1161/ATVBAHA.111.239871.
Hasan DM, Mahaney KB, Magnotta VA, Kung DK, Lawton MT, Hashimoto T, Winn HR, Saloner D, Martin A, Gahramanov S, Dósa E, Neuwelt E, Young WL. Macrophage imaging within human cerebral aneurysms wall using ferumoxytol-enhanced MRI: a pilot study. Arterioscler Thromb Vasc Biol. 2012 Apr;32(4):1032–1038.

Published In

Arterioscler Thromb Vasc Biol

DOI

EISSN

1524-4636

Publication Date

April 2012

Volume

32

Issue

4

Start / End Page

1032 / 1038

Location

United States

Related Subject Headings

  • Time Factors
  • Staining and Labeling
  • Reproducibility of Results
  • Prospective Studies
  • Predictive Value of Tests
  • Pilot Projects
  • Middle Aged
  • Male
  • Magnetic Resonance Imaging
  • Macrophages