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Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results.

Publication ,  Journal Article
Hasan, D; Chalouhi, N; Jabbour, P; Hashimoto, T
Published in: J Neuroinflammation
September 21, 2012

BACKGROUND: M1 and M2 cells are two major subsets of human macrophages that exert opposite effects on the inflammatory response. This study aims to investigate the role of macrophage M1/M2 imbalance and mast cells in the progression of human cerebral aneurysms to rupture. METHODS: Ten patients with cerebral aneurysms (five ruptured and five unruptured) underwent microsurgical clipping. During the procedure, a segment of the aneurysm dome was resected and immunostained with monoclonal antibodies for M1 cells (anti-HLA DR), M2 cells (anti-CD 163), and mast cells (anti-tryptase clone AA). A segment of the superficial temporal artery (STA) was also removed and immunostained with monoclonal antibodies for M1, M2, and mast cells. RESULTS: All ten aneurysm tissues stained positive for M1, M2, and mast cells. M1 and M2 cells were present in equal proportions in unruptured aneurysms. This contrasted with a marked predominance of M1 over M2 cells in ruptured aneurysms (p = 0.045). Mast cells were also prominently upregulated in ruptured aneurysms (p = 0.001). Few M1 and M2 cells were present in STA samples. CONCLUSIONS: M1/M2 macrophages and mast cells are found in human cerebral aneurysms; however, M1 and mast cell expression seems to markedly increase in ruptured aneurysms. These findings suggest that macrophage M1/M2 imbalance and upregulation of mast cells may have a role in the progression of cerebral aneurysms to rupture.

Duke Scholars

Published In

J Neuroinflammation

DOI

EISSN

1742-2094

Publication Date

September 21, 2012

Volume

9

Start / End Page

222

Location

England

Related Subject Headings

  • Up-Regulation
  • Temporal Arteries
  • Receptors, Cell Surface
  • Neurology & Neurosurgery
  • Middle Aged
  • Mast Cells
  • Male
  • Macrophages
  • Intracranial Aneurysm
  • Humans
 

Citation

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Hasan, D., Chalouhi, N., Jabbour, P., & Hashimoto, T. (2012). Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results. J Neuroinflammation, 9, 222. https://doi.org/10.1186/1742-2094-9-222
Hasan, David, Nohra Chalouhi, Pascal Jabbour, and Tomoki Hashimoto. “Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results.J Neuroinflammation 9 (September 21, 2012): 222. https://doi.org/10.1186/1742-2094-9-222.
Hasan, David, et al. “Macrophage imbalance (M1 vs. M2) and upregulation of mast cells in wall of ruptured human cerebral aneurysms: preliminary results.J Neuroinflammation, vol. 9, Sept. 2012, p. 222. Pubmed, doi:10.1186/1742-2094-9-222.
Journal cover image

Published In

J Neuroinflammation

DOI

EISSN

1742-2094

Publication Date

September 21, 2012

Volume

9

Start / End Page

222

Location

England

Related Subject Headings

  • Up-Regulation
  • Temporal Arteries
  • Receptors, Cell Surface
  • Neurology & Neurosurgery
  • Middle Aged
  • Mast Cells
  • Male
  • Macrophages
  • Intracranial Aneurysm
  • Humans