Skip to main content
Journal cover image

Relationship between brain and plasma carbaryl levels and cholinesterase inhibition.

Publication ,  Journal Article
Herr, DW; Mwanza, J-C; Lyke, DF; Graff, JE; Moser, VC; Padilla, S
Published in: Toxicology
October 2010

Carbaryl is a N-methylcarbamate pesticide and, like others in this class, is a reversible inhibitor of cholinesterase (ChE) enzymes. Although studied for many years, there is a surprising lack of information relating tissue levels of carbaryl with ChE activity in the same animals. The present studies were undertaken to describe the dose-response relationship about 40 min (approximate time of maximal ChE inhibition) after oral treatment in adult, post-natal day (PND) 17, and PND11 rats. Additionally, the time-course of plasma ChE activity and carbaryl levels in adult rats was determined after a 30 mg/kg dosage of carbaryl. The time-course study found that carbaryl levels could be detected in plasma 1 h after dosing, but rapidly decreased below the level of quantitation by the 2 h time point. In the dose-response studies, treatment-related increases in plasma and brain carbaryl levels were observed 40 min after dosing. Plasma levels of carbaryl increased linearly, while brain levels appeared to asymptote after 75 mg/kg carbaryl. Plasma and brain levels of carbaryl appeared to be linearly related with a slope close to 1 after various dosages (range: 1-75 mg/kg) of carbaryl at the 40 min time point. Finally, the dose-related relationship between tissue levels of carbaryl and ChE activity was described using a first order exponential decay function with an asymptote. The parameters of this function did not appear to differ between adult, PND17, or PND11 rats. This indicates that age-related differences in brain ChE inhibition by carbaryl are unlikely to be the result of greater tissue levels of the pesticide in PND11 animals. These are the first studies to report the relationship between brain and plasma tissue levels of carbaryl and ChE activity on an individual animal basis. The results of these experiments will be useful to extend physiologically-based pharmacokinetic models for carbaryl and their application in risk assessment.

Duke Scholars

Published In

Toxicology

DOI

EISSN

1879-3185

ISSN

0300-483X

Publication Date

October 2010

Volume

276

Issue

3

Start / End Page

172 / 183

Related Subject Headings

  • Toxicology
  • Tissue Distribution
  • Rats, Long-Evans
  • Rats
  • Pregnancy
  • Male
  • Female
  • Dose-Response Relationship, Drug
  • Cholinesterase Inhibitors
  • Carbaryl
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Herr, D. W., Mwanza, J.-C., Lyke, D. F., Graff, J. E., Moser, V. C., & Padilla, S. (2010). Relationship between brain and plasma carbaryl levels and cholinesterase inhibition. Toxicology, 276(3), 172–183. https://doi.org/10.1016/j.tox.2010.08.001
Herr, David W., Jean-Claude Mwanza, Danielle F. Lyke, Jaimie E. Graff, Virginia C. Moser, and Stephanie Padilla. “Relationship between brain and plasma carbaryl levels and cholinesterase inhibition.Toxicology 276, no. 3 (October 2010): 172–83. https://doi.org/10.1016/j.tox.2010.08.001.
Herr DW, Mwanza J-C, Lyke DF, Graff JE, Moser VC, Padilla S. Relationship between brain and plasma carbaryl levels and cholinesterase inhibition. Toxicology. 2010 Oct;276(3):172–83.
Herr, David W., et al. “Relationship between brain and plasma carbaryl levels and cholinesterase inhibition.Toxicology, vol. 276, no. 3, Oct. 2010, pp. 172–83. Epmc, doi:10.1016/j.tox.2010.08.001.
Herr DW, Mwanza J-C, Lyke DF, Graff JE, Moser VC, Padilla S. Relationship between brain and plasma carbaryl levels and cholinesterase inhibition. Toxicology. 2010 Oct;276(3):172–183.
Journal cover image

Published In

Toxicology

DOI

EISSN

1879-3185

ISSN

0300-483X

Publication Date

October 2010

Volume

276

Issue

3

Start / End Page

172 / 183

Related Subject Headings

  • Toxicology
  • Tissue Distribution
  • Rats, Long-Evans
  • Rats
  • Pregnancy
  • Male
  • Female
  • Dose-Response Relationship, Drug
  • Cholinesterase Inhibitors
  • Carbaryl