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Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection.

Publication ,  Journal Article
Raymer, JH; Sparacino, CM; Velez, GR; Padilla, S; MacPhail, RC; Crofton, KM
Published in: Journal of chromatography
September 1993

A modified method for the derivatization and determination of acrylamide as 2-bromopropenamide by gas chromatography-electron-capture detection was developed and applied to serum and sciatic nerve from rats. The method was accurate and precise over the calibration range 2.24-7.47 micrograms/ml in serum diluted 1:125 and 4-122 micrograms/g in sciatic nerve homogenate (5 mg/ml). limits of detection were estimated to be 1200 ng/ml in undiluted serum and 3 micrograms/g in intact sciatic nerve. The use of less dilute samples to allow for lower limits of detection appears feasible. The time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 h for serum and sciatic nerve, respectively. A dose-effect relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum (microgram/ml) were approximately the same as in sciatic nerve (microgram/g).

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Published In

Journal of chromatography

DOI

Publication Date

September 1993

Volume

619

Issue

2

Start / End Page

223 / 234

Related Subject Headings

  • Sciatic Nerve
  • Rats
  • Male
  • Magnetic Resonance Spectroscopy
  • Half-Life
  • Gas Chromatography-Mass Spectrometry
  • Chromatography, Gas
  • Animals
  • Acrylamides
  • Acrylamide
 

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Raymer, J. H., Sparacino, C. M., Velez, G. R., Padilla, S., MacPhail, R. C., & Crofton, K. M. (1993). Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection. Journal of Chromatography, 619(2), 223–234. https://doi.org/10.1016/0378-4347(93)80111-g
Raymer, J. H., C. M. Sparacino, G. R. Velez, S. Padilla, R. C. MacPhail, and K. M. Crofton. “Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection.Journal of Chromatography 619, no. 2 (September 1993): 223–34. https://doi.org/10.1016/0378-4347(93)80111-g.
Raymer JH, Sparacino CM, Velez GR, Padilla S, MacPhail RC, Crofton KM. Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection. Journal of chromatography. 1993 Sep;619(2):223–34.
Raymer, J. H., et al. “Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection.Journal of Chromatography, vol. 619, no. 2, Sept. 1993, pp. 223–34. Epmc, doi:10.1016/0378-4347(93)80111-g.
Raymer JH, Sparacino CM, Velez GR, Padilla S, MacPhail RC, Crofton KM. Determination of acrylamide in rat serum and sciatic nerve by gas chromatography-electron-capture detection. Journal of chromatography. 1993 Sep;619(2):223–234.

Published In

Journal of chromatography

DOI

Publication Date

September 1993

Volume

619

Issue

2

Start / End Page

223 / 234

Related Subject Headings

  • Sciatic Nerve
  • Rats
  • Male
  • Magnetic Resonance Spectroscopy
  • Half-Life
  • Gas Chromatography-Mass Spectrometry
  • Chromatography, Gas
  • Animals
  • Acrylamides
  • Acrylamide