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Factors in standardizing automated cholinesterase assays.

Publication ,  Journal Article
Wilson, BW; Padilla, S; Henderson, JD; Brimijoin, S; Dass, PD; Elliot, G; Jaeger, B; Lanz, D; Pearson, R; Spies, R
Published in: Journal of toxicology and environmental health
June 1996

A scientific panel assembled by the U.S. Environmental Protection Agency (EPA) determined that variability in cholinesterase (ChE) activities in the agency's pesticide/animal study database likely was due to a lack of accepted guidelines for ChE methodology. A series of trials was held in which participating laboratories measured ChE activity in blood and brain samples from untreated and pesticide-treated rats using a colorimetric assay method. The degree of inhibition of ChE activity in plasma and brain samples compared to controls was consistent among most of the laboratories. The ChE activity in erythrocyte samples differed more between laboratories due to a high blank, low erythrocyte AChE activity and hemoglobin absorption at the wavelength of the assay. Strategies are suggested for minimizing the variability of ChE activity in hemoglobin-rich samples.

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Published In

Journal of toxicology and environmental health

DOI

ISSN

0098-4108

Publication Date

June 1996

Volume

48

Issue

2

Start / End Page

187 / 195

Related Subject Headings

  • United States Environmental Protection Agency
  • United States
  • Toxicology
  • Reproducibility of Results
  • Rats
  • Pilot Projects
  • Niacin
  • Insecticides
  • Hydrolysis
  • Guidelines as Topic
 

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Wilson, B. W., Padilla, S., Henderson, J. D., Brimijoin, S., Dass, P. D., Elliot, G., … Spies, R. (1996). Factors in standardizing automated cholinesterase assays. Journal of Toxicology and Environmental Health, 48(2), 187–195. https://doi.org/10.1080/009841096161429
Wilson, B. W., S. Padilla, J. D. Henderson, S. Brimijoin, P. D. Dass, G. Elliot, B. Jaeger, D. Lanz, R. Pearson, and R. Spies. “Factors in standardizing automated cholinesterase assays.Journal of Toxicology and Environmental Health 48, no. 2 (June 1996): 187–95. https://doi.org/10.1080/009841096161429.
Wilson BW, Padilla S, Henderson JD, Brimijoin S, Dass PD, Elliot G, et al. Factors in standardizing automated cholinesterase assays. Journal of toxicology and environmental health. 1996 Jun;48(2):187–95.
Wilson, B. W., et al. “Factors in standardizing automated cholinesterase assays.Journal of Toxicology and Environmental Health, vol. 48, no. 2, June 1996, pp. 187–95. Epmc, doi:10.1080/009841096161429.
Wilson BW, Padilla S, Henderson JD, Brimijoin S, Dass PD, Elliot G, Jaeger B, Lanz D, Pearson R, Spies R. Factors in standardizing automated cholinesterase assays. Journal of toxicology and environmental health. 1996 Jun;48(2):187–195.

Published In

Journal of toxicology and environmental health

DOI

ISSN

0098-4108

Publication Date

June 1996

Volume

48

Issue

2

Start / End Page

187 / 195

Related Subject Headings

  • United States Environmental Protection Agency
  • United States
  • Toxicology
  • Reproducibility of Results
  • Rats
  • Pilot Projects
  • Niacin
  • Insecticides
  • Hydrolysis
  • Guidelines as Topic