Skip to main content
Journal cover image

Vitamin K-dependent protein S in Leydig cells of human testis.

Publication ,  Journal Article
Malm, J; He, XH; Bjartell, A; Shen, L; Abrahamsson, PA; Dahlbäck, B
Published in: Biochem J
September 15, 1994

Protein S is an anticoagulant plasma protein, functioning as a cofactor to activated protein C in the regulation of blood coagulation. In addition, protein S forms a complex with the complement regulatory protein, C4b-binding protein. Protein S is unique among the vitamin K-dependent proteins in being structurally similar to androgen binding proteins. Protein S immunoreactivity was demonstrated in Leydig cells of human testis. In Northern blotting experiments, the presence of protein S mRNA in human testis tissue could be shown. In situ hybridization experiments localized protein S mRNA to the Leydig cells, demonstrating transcription of the protein S gene in these cells. Five protein S clones were isolated from a human testis cDNA library, partially sequenced and characterized by restriction enzyme mapping. Three unique clones contained information for the entire coding sequence and approximately two-thirds of the 5' and 3' non-coding sequences. The results indicate the nucleotide sequences of testis and liver protein S mRNA to be identical. No binding of androgens to protein S could be demonstrated. In conclusion, we demonstrate the presence of protein S immunoreactivity as well as protein S mRNA in the Leydig cells of human testis. These results suggest local synthesis of protein S in Leydig cells of human testis which may be functionally important for local anticoagulation.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

September 15, 1994

Volume

302 ( Pt 3)

Issue

Pt 3

Start / End Page

845 / 850

Location

England

Related Subject Headings

  • RNA, Messenger
  • Protein S
  • Oligonucleotide Probes
  • Molecular Sequence Data
  • Male
  • Leydig Cells
  • In Situ Hybridization
  • Immunohistochemistry
  • Humans
  • DNA, Complementary
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Malm, J., He, X. H., Bjartell, A., Shen, L., Abrahamsson, P. A., & Dahlbäck, B. (1994). Vitamin K-dependent protein S in Leydig cells of human testis. Biochem J, 302 ( Pt 3)(Pt 3), 845–850. https://doi.org/10.1042/bj3020845
Malm, J., X. H. He, A. Bjartell, L. Shen, P. A. Abrahamsson, and B. Dahlbäck. “Vitamin K-dependent protein S in Leydig cells of human testis.Biochem J 302 ( Pt 3), no. Pt 3 (September 15, 1994): 845–50. https://doi.org/10.1042/bj3020845.
Malm J, He XH, Bjartell A, Shen L, Abrahamsson PA, Dahlbäck B. Vitamin K-dependent protein S in Leydig cells of human testis. Biochem J. 1994 Sep 15;302 ( Pt 3)(Pt 3):845–50.
Malm, J., et al. “Vitamin K-dependent protein S in Leydig cells of human testis.Biochem J, vol. 302 ( Pt 3), no. Pt 3, Sept. 1994, pp. 845–50. Pubmed, doi:10.1042/bj3020845.
Malm J, He XH, Bjartell A, Shen L, Abrahamsson PA, Dahlbäck B. Vitamin K-dependent protein S in Leydig cells of human testis. Biochem J. 1994 Sep 15;302 ( Pt 3)(Pt 3):845–850.
Journal cover image

Published In

Biochem J

DOI

ISSN

0264-6021

Publication Date

September 15, 1994

Volume

302 ( Pt 3)

Issue

Pt 3

Start / End Page

845 / 850

Location

England

Related Subject Headings

  • RNA, Messenger
  • Protein S
  • Oligonucleotide Probes
  • Molecular Sequence Data
  • Male
  • Leydig Cells
  • In Situ Hybridization
  • Immunohistochemistry
  • Humans
  • DNA, Complementary