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Role of inositol 1,4,5-triphosphate signalling in gravitropic and phototropic gene expression.

Publication ,  Journal Article
Salinas-Mondragon, RE; Kajla, JD; Perera, IY; Brown, CS; Sederoff, HW
Published in: Plant, cell & environment
December 2010

Plants sense light and gravity to orient their direction of growth. One common component in the early events of both phototropic and gravitropic signal transduction is activation of phospholipase C (PLC), which leads to an increase in inositol 1,4,5-triphosphate (InsP(3)) levels. The InsP(3) signal is terminated by hydrolysis of InsP(3) through inositolpolyphosphate-5-phosphatases (InsP 5-ptases). Arabidopsis plants expressing a heterologous InsP 5-ptase have low basal InsP(3) levels and exhibit reduced gravitropic and phototropic bending. Downstream effects of InsP(3)-mediated signalling are not understood. We used comparative transcript profiling to characterize gene expression changes in gravity- or light-stimulated Arabidopsis root apices that were manipulated in their InsP(3) metabolism either through inhibition of PLC activity or expression of InsP 5-ptase. We identified InsP(3)-dependent and InsP(3)-independent co-regulated gene sets in response to gravity or light stimulation. Inhibition of PLC activity in wild-type plants caused similar changes in transcript abundance in response to gravitropic and phototropic stimulation as in the transgenic lines. Therefore, we conclude that changes in gene expression in response to gravitropic and phototropic stimulation are mediated by two signal transduction pathways that vary in their dependence on changes in InsP(3).

Duke Scholars

Published In

Plant, cell & environment

DOI

EISSN

1365-3040

ISSN

0140-7791

Publication Date

December 2010

Volume

33

Issue

12

Start / End Page

2041 / 2055

Related Subject Headings

  • Type C Phospholipases
  • Signal Transduction
  • Plants, Genetically Modified
  • Plant Roots
  • Plant Biology & Botany
  • Phototropism
  • Inositol 1,4,5-Trisphosphate
  • Hypocotyl
  • Gravitropism
  • Gene Expression Regulation, Plant
 

Citation

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MLA
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Salinas-Mondragon, R. E., Kajla, J. D., Perera, I. Y., Brown, C. S., & Sederoff, H. W. (2010). Role of inositol 1,4,5-triphosphate signalling in gravitropic and phototropic gene expression. Plant, Cell & Environment, 33(12), 2041–2055. https://doi.org/10.1111/j.1365-3040.2010.02204.x
Salinas-Mondragon, Raul E., Jyoti D. Kajla, Imara Y. Perera, Christopher S. Brown, and Heike Winter Sederoff. “Role of inositol 1,4,5-triphosphate signalling in gravitropic and phototropic gene expression.Plant, Cell & Environment 33, no. 12 (December 2010): 2041–55. https://doi.org/10.1111/j.1365-3040.2010.02204.x.
Salinas-Mondragon RE, Kajla JD, Perera IY, Brown CS, Sederoff HW. Role of inositol 1,4,5-triphosphate signalling in gravitropic and phototropic gene expression. Plant, cell & environment. 2010 Dec;33(12):2041–55.
Salinas-Mondragon, Raul E., et al. “Role of inositol 1,4,5-triphosphate signalling in gravitropic and phototropic gene expression.Plant, Cell & Environment, vol. 33, no. 12, Dec. 2010, pp. 2041–55. Epmc, doi:10.1111/j.1365-3040.2010.02204.x.
Salinas-Mondragon RE, Kajla JD, Perera IY, Brown CS, Sederoff HW. Role of inositol 1,4,5-triphosphate signalling in gravitropic and phototropic gene expression. Plant, cell & environment. 2010 Dec;33(12):2041–2055.
Journal cover image

Published In

Plant, cell & environment

DOI

EISSN

1365-3040

ISSN

0140-7791

Publication Date

December 2010

Volume

33

Issue

12

Start / End Page

2041 / 2055

Related Subject Headings

  • Type C Phospholipases
  • Signal Transduction
  • Plants, Genetically Modified
  • Plant Roots
  • Plant Biology & Botany
  • Phototropism
  • Inositol 1,4,5-Trisphosphate
  • Hypocotyl
  • Gravitropism
  • Gene Expression Regulation, Plant