Skip to main content
Journal cover image

Isolation and characterization of stem cells from the human parathyroid gland.

Publication ,  Journal Article
Shih, Y-RV; Kuo, TK; Yang, A-H; Lee, OK; Lee, C-H
Published in: Cell Prolif
August 2009

OBJECTIVES: Somatic stem cells can be obtained from a variety of adult human tissues. However, it was not clear whether human parathyroid glands, which secrete parathyroid hormones and are essential in maintaining homeostasis levels of calcium ions in the circulation, contained stem cells. We aimed to investigate the possibility of isolating such parathyroid-derived stem cells (PDSC). MATERIALS AND METHODS: Surgically removed parathyroid glands were obtained with informed consent. Cell cytogenetics was used to observe chromosomal abnormalities. Surface phenotypes were characterized by flow cytometry. Telomerase repeat amplification protocol (TRAP) assay was performed to observe the telomerase activity. RT-PCR and real-time PCR was was used to detect gene expressions. Real-time calcium uptake imaging was performed for extent of calcium uptake and transmission electron microscopy and immunofluorecent staining for smooth muscle actin. RESULTS: After enzymatic digestion and primary culture, plastic-adherent, fibroblast-like cells appeared in culture and a morphologically homogeneous population was derived from subsequent limiting dilution and clonal expansion. Karyotyping was normal and doubling time of clonal cell growth was estimated to be 70.7 +/- 14.5 h (mean +/- standard deviation). The surface phenotype of the cells was positive for CD73, CD166, CD29, CD49a, CD49b, CD49d, CD44, CD105, and MHC class I, and negative for CD34, CD133, CD117, CD114, CD31, CD62P, EGF-R, ICAM-3, CD26, CXCR4, CD106, CD90 and MHC class II, similar to mesenchymal stem cells (MSC). Detectable levels of telomerase activity along with pluripotency Sall4 gene expression were observed from the isolated PDSCs. Expression of calcium-sensing receptor gene along with alpha-smooth muscle actin was induced and cellular uptake of extracellular calcium ions was observed. Furthermore, PDSCs possessed osteogenic, chondrogenic and adipogenic differentiation potentials. CONCLUSIONS: Our results reveal that PDSCs were similar phenotypically to MSCs and further studies are needed to formulate induction conditions to differentiate PDSCs into parathyroid hormone-secreting chief cells.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell Prolif

DOI

EISSN

1365-2184

Publication Date

August 2009

Volume

42

Issue

4

Start / End Page

461 / 470

Location

England

Related Subject Headings

  • Transcription Factors
  • Telomerase
  • Stem Cells
  • Receptors, Calcium-Sensing
  • Phenotype
  • Parathyroid Glands
  • Osteogenesis
  • Oncology & Carcinogenesis
  • Humans
  • Gene Expression
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Shih, Y.-R., Kuo, T. K., Yang, A.-H., Lee, O. K., & Lee, C.-H. (2009). Isolation and characterization of stem cells from the human parathyroid gland. Cell Prolif, 42(4), 461–470. https://doi.org/10.1111/j.1365-2184.2009.00614.x
Shih, Y. -. R. V., T. K. Kuo, A. -. H. Yang, O. K. Lee, and C. -. H. Lee. “Isolation and characterization of stem cells from the human parathyroid gland.Cell Prolif 42, no. 4 (August 2009): 461–70. https://doi.org/10.1111/j.1365-2184.2009.00614.x.
Shih Y-RV, Kuo TK, Yang A-H, Lee OK, Lee C-H. Isolation and characterization of stem cells from the human parathyroid gland. Cell Prolif. 2009 Aug;42(4):461–70.
Shih, Y. .. R. V., et al. “Isolation and characterization of stem cells from the human parathyroid gland.Cell Prolif, vol. 42, no. 4, Aug. 2009, pp. 461–70. Pubmed, doi:10.1111/j.1365-2184.2009.00614.x.
Shih Y-RV, Kuo TK, Yang A-H, Lee OK, Lee C-H. Isolation and characterization of stem cells from the human parathyroid gland. Cell Prolif. 2009 Aug;42(4):461–470.
Journal cover image

Published In

Cell Prolif

DOI

EISSN

1365-2184

Publication Date

August 2009

Volume

42

Issue

4

Start / End Page

461 / 470

Location

England

Related Subject Headings

  • Transcription Factors
  • Telomerase
  • Stem Cells
  • Receptors, Calcium-Sensing
  • Phenotype
  • Parathyroid Glands
  • Osteogenesis
  • Oncology & Carcinogenesis
  • Humans
  • Gene Expression