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Multimodal optical and Gd-based nanoparticles for imaging in inflammatory arthritis.

Publication ,  Journal Article
Kim, JS; An, H; Rieter, WJ; Esserman, D; Taylor-Pashow, KML; Sartor, RB; Lin, W; Tarrant, TK
Published in: Clin Exp Rheumatol
2009

OBJECTIVES: This report documents a multimodal nanoparticle (MNP) contrast agent, containing embedded luminophores and surface-immobilized gadolinium chelates, as a contrast agent of inflamed synovium in a collagen induced arthritis (CIA) model. METHODS: DBA-1J mice were immunized for CIA and imaged after disease onset by two independent modalities. After intravenous administration of MNP contrast, optical and magnetic resonance images were obtained and clinical disease was scored, which was followed by processing of hindlimbs for immunofluorescence and confocal microscopy. RESULTS: We show a correlation between disease severity and MNP optical luminescence that is dose dependent. Immunofluorescence of hindlimb sections reveal that MNP-labeled cells are monocytes/macrophages within the inflamed synovium. Magnetic resonance (MR) relaxation time maps, which determine the quantitative measure of T1 and T2 values at each imaging voxel, demonstrated a decreasing T2 signal in actively inflamed joints that was more pronounced earlier rather than later during disease. CONCLUSIONS: MNPs containing surface-immobilized gadolinium chelates and embedded luminophores are potential dual-modality contrast agents in inflammatory arthritis and localize to monocytes/macrophages within inflamed synovium.

Duke Scholars

Published In

Clin Exp Rheumatol

ISSN

0392-856X

Publication Date

2009

Volume

27

Issue

4

Start / End Page

580 / 586

Location

Italy

Related Subject Headings

  • Synovitis
  • Monocytes
  • Microscopy, Confocal
  • Mice, Inbred DBA
  • Mice
  • Metal Nanoparticles
  • Magnetic Resonance Imaging
  • Macrophages
  • Injections, Intravenous
  • Hindlimb
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kim, J. S., An, H., Rieter, W. J., Esserman, D., Taylor-Pashow, K. M. L., Sartor, R. B., … Tarrant, T. K. (2009). Multimodal optical and Gd-based nanoparticles for imaging in inflammatory arthritis. Clin Exp Rheumatol, 27(4), 580–586.
Kim, J. S., H. An, W. J. Rieter, D. Esserman, K. M. L. Taylor-Pashow, R. B. Sartor, W. Lin, and T. K. Tarrant. “Multimodal optical and Gd-based nanoparticles for imaging in inflammatory arthritis.Clin Exp Rheumatol 27, no. 4 (2009): 580–86.
Kim JS, An H, Rieter WJ, Esserman D, Taylor-Pashow KML, Sartor RB, et al. Multimodal optical and Gd-based nanoparticles for imaging in inflammatory arthritis. Clin Exp Rheumatol. 2009;27(4):580–6.
Kim, J. S., et al. “Multimodal optical and Gd-based nanoparticles for imaging in inflammatory arthritis.Clin Exp Rheumatol, vol. 27, no. 4, 2009, pp. 580–86.
Kim JS, An H, Rieter WJ, Esserman D, Taylor-Pashow KML, Sartor RB, Lin W, Tarrant TK. Multimodal optical and Gd-based nanoparticles for imaging in inflammatory arthritis. Clin Exp Rheumatol. 2009;27(4):580–586.

Published In

Clin Exp Rheumatol

ISSN

0392-856X

Publication Date

2009

Volume

27

Issue

4

Start / End Page

580 / 586

Location

Italy

Related Subject Headings

  • Synovitis
  • Monocytes
  • Microscopy, Confocal
  • Mice, Inbred DBA
  • Mice
  • Metal Nanoparticles
  • Magnetic Resonance Imaging
  • Macrophages
  • Injections, Intravenous
  • Hindlimb