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Abstract 140: Effect of Transendocardial Autologous Cardiac Stem Cells and Bone Marrow Mesenchymal Stem Cells to Reduce Infarct Size and Restore Cardiac Function in a Heart Failure Swine Model

Publication ,  Journal Article
Suncion, VY; Karantalis, V; Bagno, LL; McCall, F; Wang, B; Golpanian, S; Rodriguez, JE; Rosado, M; Valdes, D; Gomes, S; Da Silva, J; Premer, C ...
Published in: Circulation Research
July 18, 2014

A cell combination of human mesenchymal stem cells (MSCs) and c-kit+ cardiac stem cells (CSCs) improves left ventricular (LV) performance to a greater degree than MSCs alone in post myocardial infarction swine. To advance the development of cell combination therapy, we administered autologous swine cells, and tested the hypothesis that transendocardial autologous CSCs/MSCs produces greater improvement of performance than MSCs in a rigorous model of heart failure due to post infarct LV remodeling. Gottingen mini-swine (n=28) underwent LAD coronary artery occlusion followed by reperfusion, and allowed to undergo LV remodeling for 90 days. Autologous MSCs were amplified from bone marrow and CSCs from right ventricular biopsies in each swine, and injections of either CSC/MSC combo (1M/200M, n=7), MSCs (200M, n=7), or placebo (Plasmalyte, n=6) were injected to the infarct-border zone via the NOGA system. Cardiac MRI and pressure volume loops were obtained before and after therapy. Both cell groups had substantially reduced scar size (Combo –37.2.9± 5.4% vs MSCs –38.8±7.5% vs placebo −7.2±6.3, P=0.0001) and increased viable tissue (Combo +30.9±7% vs MSCs +41.8±10.5% vs placebo +7.7±4.5, P<0.0001) relative to placebo. Ejection Fraction (EF) improved only in the Combo group (Combo +7.0±2.8 vs MSCs +3.4±1.3 vs placebo +1.2±1.6 EF units, P=0.04). Accompanying this EF restoration was a substantial improvement in the Combo group in stroke volume (Combo +47.2±11.1% vs MSCs +32.6±12.0% vs placebo +10.8±4.5, P<0.0001), cardiac output (Combo +35.9±7.6% vs MSCs 41.9±26.5% vs placebo −16.4±6.6%, P=0.01) and diastolic strain rate (Combo +18.9±8.6% vs MSCs 14.0±8.8% vs placebo −14.9±9.5%, P=0.03). Combination cell therapy and MSCs alone dramatically reduce scar size in a swine model of chronic ischemic cardiomyopathy. In contrast, combination therapy has much greater impact on functional recovery, increasing EF to [near normal] levels. These findings illustrate that interactions between ckit+ CSCs and MSCs result in substantial enhancement in cardiac performance, establish the safety of autologous cell combination strategies, and support the development of an advanced second generation cell therapeutic product.

Duke Scholars

Published In

Circulation Research

DOI

EISSN

1524-4571

ISSN

0009-7330

Publication Date

July 18, 2014

Volume

115

Issue

suppl_1

Publisher

Ovid Technologies (Wolters Kluwer Health)

Related Subject Headings

  • Cardiovascular System & Hematology
  • 3202 Clinical sciences
  • 3201 Cardiovascular medicine and haematology
  • 1103 Clinical Sciences
  • 1102 Cardiorespiratory Medicine and Haematology
 

Citation

APA
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ICMJE
MLA
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Suncion, V. Y., Karantalis, V., Bagno, L. L., McCall, F., Wang, B., Golpanian, S., … Hare, J. M. (2014). Abstract 140: Effect of Transendocardial Autologous Cardiac Stem Cells and Bone Marrow Mesenchymal Stem Cells to Reduce Infarct Size and Restore Cardiac Function in a Heart Failure Swine Model. Circulation Research, 115(suppl_1). https://doi.org/10.1161/res.115.suppl_1.140
Suncion, Viky Y., Vasileios Karantalis, Luiza L. Bagno, Frederic McCall, Bo Wang, Samuel Golpanian, Jose E. Rodriguez, et al. “Abstract 140: Effect of Transendocardial Autologous Cardiac Stem Cells and Bone Marrow Mesenchymal Stem Cells to Reduce Infarct Size and Restore Cardiac Function in a Heart Failure Swine Model.” Circulation Research 115, no. suppl_1 (July 18, 2014). https://doi.org/10.1161/res.115.suppl_1.140.
Suncion, Viky Y., et al. “Abstract 140: Effect of Transendocardial Autologous Cardiac Stem Cells and Bone Marrow Mesenchymal Stem Cells to Reduce Infarct Size and Restore Cardiac Function in a Heart Failure Swine Model.” Circulation Research, vol. 115, no. suppl_1, Ovid Technologies (Wolters Kluwer Health), July 2014. Crossref, doi:10.1161/res.115.suppl_1.140.
Suncion VY, Karantalis V, Bagno LL, McCall F, Wang B, Golpanian S, Rodriguez JE, Rosado M, Valdes D, Gomes S, Da Silva J, Premer C, Morales A, Balkan W, Mushtaq M, Zambrano JP, Heldman AW, Hare JM. Abstract 140: Effect of Transendocardial Autologous Cardiac Stem Cells and Bone Marrow Mesenchymal Stem Cells to Reduce Infarct Size and Restore Cardiac Function in a Heart Failure Swine Model. Circulation Research. Ovid Technologies (Wolters Kluwer Health); 2014 Jul 18;115(suppl_1).

Published In

Circulation Research

DOI

EISSN

1524-4571

ISSN

0009-7330

Publication Date

July 18, 2014

Volume

115

Issue

suppl_1

Publisher

Ovid Technologies (Wolters Kluwer Health)

Related Subject Headings

  • Cardiovascular System & Hematology
  • 3202 Clinical sciences
  • 3201 Cardiovascular medicine and haematology
  • 1103 Clinical Sciences
  • 1102 Cardiorespiratory Medicine and Haematology