Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans
View expanded cover CRISPR-Cas Methods pp 357–370Cite as Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans Hyun-Min Kim & Xiaojuan Zhang Protocol First Online: 31 July 2021 759 Accesses Part of the Springer Protocols Handbooks book series (SPH) Abstract CRISPR-Cas9 is the current choice for genome editing for its versatility and specificity. The Cas9 endonuclease makes double-strand breaks at the target sites, which are repaired via non-homologous end-joining or homologous recombination. So far, various genome editing methods using CRISPR-Cas have been developed for Caenorhabditis elegans. However, repairing a double-strand break via homologous recombination is a crucial step to modify a genome in an error-free manner. Here, we focus on a procedure on how to prepare repair templates for precise genome editing via homologous recombination in C. elegans, which applies to a variety of CRISPR-Cas methods.