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Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans

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Kim, H; Zhang, X
July 31, 2021

View expanded cover CRISPR-Cas Methods pp 357–370Cite as Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans Hyun-Min Kim & Xiaojuan Zhang Protocol First Online: 31 July 2021 759 Accesses Part of the Springer Protocols Handbooks book series (SPH) Abstract CRISPR-Cas9 is the current choice for genome editing for its versatility and specificity. The Cas9 endonuclease makes double-strand breaks at the target sites, which are repaired via non-homologous end-joining or homologous recombination. So far, various genome editing methods using CRISPR-Cas have been developed for Caenorhabditis elegans. However, repairing a double-strand break via homologous recombination is a crucial step to modify a genome in an error-free manner. Here, we focus on a procedure on how to prepare repair templates for precise genome editing via homologous recombination in C. elegans, which applies to a variety of CRISPR-Cas methods.

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July 31, 2021

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Springer Nature. Humana, New York
 

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Kim, H., & Zhang, X. (2021). Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans. Springer Nature. Humana, New York. https://doi.org/10.1007/978-1-0716-1657-4_24
Kim, Hyun, and Xiaojuan Zhang. Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans. Springer Nature. Humana, New York, 2021. https://doi.org/10.1007/978-1-0716-1657-4_24.
Kim, Hyun, and Xiaojuan Zhang. Design of Repair Templates for CRISPR-Cas9-Triggered Homologous Recombination in Caenorhabditis elegans. Springer Nature. Humana, New York, 2021. Manual, doi:10.1007/978-1-0716-1657-4_24.

DOI

Publication Date

July 31, 2021

Publisher

Springer Nature. Humana, New York