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Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury.

Publication ,  Journal Article
Albright, M; Guttenberg, MA; Tighe, RM
Published in: Methods Mol Biol
2022

Ozone (O3), a criterion air pollutant produced as a product of internal combustion, generates increased inflammation, lung permeability, and airway hyperreactivity when exposed to rodents in laboratory settings. Airway hyperreactivity is defined as an exaggerated acute obstructive response of the airways to one or more nonspecific stimuli. Lung permeability is a measure of barrier functions that separate internal and external environments to limit access of pathogens and other noxious material. By modeling in vivo O3 exposure in rodents, this allows investigators to explore pulmonary and nonpulmonary O3 effects as a means of understanding its impact on human health and lung function. Furthermore, direct effects of O3 on epithelial permeability can be defined using in vitro exposures to airway epithelial cells. This chapter will focus on methods of generating O3 and then exposing rodents and cultured epithelial cells in laboratory settings.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2022

Volume

2506

Start / End Page

67 / 81

Location

United States

Related Subject Headings

  • Ozone
  • Lung
  • Humans
  • Developmental Biology
  • Bronchoalveolar Lavage Fluid
  • Asthma
  • Air Pollutants
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
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ICMJE
MLA
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Albright, M., Guttenberg, M. A., & Tighe, R. M. (2022). Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury. Methods Mol Biol, 2506, 67–81. https://doi.org/10.1007/978-1-0716-2364-0_5
Albright, Michaela, Marissa A. Guttenberg, and Robert M. Tighe. “Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury.Methods Mol Biol 2506 (2022): 67–81. https://doi.org/10.1007/978-1-0716-2364-0_5.
Albright M, Guttenberg MA, Tighe RM. Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury. Methods Mol Biol. 2022;2506:67–81.
Albright, Michaela, et al. “Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury.Methods Mol Biol, vol. 2506, 2022, pp. 67–81. Pubmed, doi:10.1007/978-1-0716-2364-0_5.
Albright M, Guttenberg MA, Tighe RM. Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury. Methods Mol Biol. 2022;2506:67–81.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2022

Volume

2506

Start / End Page

67 / 81

Location

United States

Related Subject Headings

  • Ozone
  • Lung
  • Humans
  • Developmental Biology
  • Bronchoalveolar Lavage Fluid
  • Asthma
  • Air Pollutants
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology