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Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution.

Publication ,  Journal Article
Ding, Y; Lee, J; Ma, J; Sung, K; Yokota, T; Singh, N; Dooraghi, M; Abiri, P; Wang, Y; Kulkarni, RP; Nakano, A; Nguyen, TP; Fei, P; Hsiai, TK
Published in: Sci Rep
February 6, 2017

Light-sheet fluorescence microscopy (LSFM) serves to advance developmental research and regenerative medicine. Coupled with the paralleled advances in fluorescence-friendly tissue clearing technique, our cardiac LSFM enables dual-sided illumination to rapidly uncover the architecture of murine hearts over 10 by 10 by 10 mm3 in volume; thereby allowing for localizing progenitor differentiation to the cardiomyocyte lineage and AAV9-mediated expression of exogenous transmembrane potassium channels with high contrast and resolution. Without the steps of stitching image columns, pivoting the light-sheet and sectioning the heart mechanically, we establish a holistic strategy for 3-dimentional reconstruction of the "digital murine heart" to assess aberrant cardiac structures as well as the spatial distribution of the cardiac lineages in neonates and ion-channels in adults.

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

February 6, 2017

Volume

7

Start / End Page

42209

Location

England

Related Subject Headings

  • Proteins
  • Potassium Channels
  • Myocardium
  • Microscopy, Fluorescence
  • Mice
  • Imaging, Three-Dimensional
  • Heart Ventricles
  • Green Fluorescent Proteins
  • Fluorescence
  • Cell Lineage
 

Citation

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Ding, Y., Lee, J., Ma, J., Sung, K., Yokota, T., Singh, N., … Hsiai, T. K. (2017). Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution. Sci Rep, 7, 42209. https://doi.org/10.1038/srep42209
Ding, Yichen, Juhyun Lee, Jianguo Ma, Kevin Sung, Tomohiro Yokota, Neha Singh, Mojdeh Dooraghi, et al. “Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution.Sci Rep 7 (February 6, 2017): 42209. https://doi.org/10.1038/srep42209.
Ding Y, Lee J, Ma J, Sung K, Yokota T, Singh N, et al. Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution. Sci Rep. 2017 Feb 6;7:42209.
Ding, Yichen, et al. “Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution.Sci Rep, vol. 7, Feb. 2017, p. 42209. Pubmed, doi:10.1038/srep42209.
Ding Y, Lee J, Ma J, Sung K, Yokota T, Singh N, Dooraghi M, Abiri P, Wang Y, Kulkarni RP, Nakano A, Nguyen TP, Fei P, Hsiai TK. Light-sheet fluorescence imaging to localize cardiac lineage and protein distribution. Sci Rep. 2017 Feb 6;7:42209.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

February 6, 2017

Volume

7

Start / End Page

42209

Location

England

Related Subject Headings

  • Proteins
  • Potassium Channels
  • Myocardium
  • Microscopy, Fluorescence
  • Mice
  • Imaging, Three-Dimensional
  • Heart Ventricles
  • Green Fluorescent Proteins
  • Fluorescence
  • Cell Lineage