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Diurnal variation of circulating human myeloid progenitor cells.

Publication ,  Journal Article
Ross, DD; Pollak, A; Akman, SA; Bachur, NR
Published in: Exp Hematol
August 1980

We investigated whether human circulating myeloid progenitor cells (CFU(c)) exhibit diurnal variation. Peripheral venous blood samples were drawn every 3 h from each of the nine healthy male volunteers. Blood mononuclear cells were harvested by the Ficoll-Hypaque method. CFU(c) content of blood was determined by the soft agar cloning assay. Human placental conditioned medium (HPCM) was used as colony stimulating factor. We found a 24 h cyclic variation of colonies/10(5) mononuclear cells plated with a maximum at 9 a.m. (P < 0.01). Analysis of the colonies by in situ staining indicated that this variation could be attributed almost entirely to colonies composed of eosinophilic cells (representative of CFU(eos)). Neutrophil/macrophage colonies (representative of CFU(G,M)) exhibited a trend toward a 9 a.m. maximum, but this variation was not statistically significant. We conclude that time of blood collection should be standardized in experiments measuring human peripheral blood CFU(c).

Published In

Exp Hematol

ISSN

0301-472X

Publication Date

August 1980

Volume

8

Issue

7

Start / End Page

954 / 960

Location

Netherlands

Related Subject Headings

  • Neutrophils
  • Myeloid Progenitor Cells
  • Male
  • Macrophages
  • Immunology
  • Humans
  • Colony-Forming Units Assay
  • Circadian Rhythm
  • Cells, Cultured
  • 3201 Cardiovascular medicine and haematology
 

Citation

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ICMJE
MLA
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Ross, D. D., Pollak, A., Akman, S. A., & Bachur, N. R. (1980). Diurnal variation of circulating human myeloid progenitor cells. Exp Hematol, 8(7), 954–960.
Ross, D. D., A. Pollak, S. A. Akman, and N. R. Bachur. “Diurnal variation of circulating human myeloid progenitor cells.Exp Hematol 8, no. 7 (August 1980): 954–60.
Ross DD, Pollak A, Akman SA, Bachur NR. Diurnal variation of circulating human myeloid progenitor cells. Exp Hematol. 1980 Aug;8(7):954–60.
Ross, D. D., et al. “Diurnal variation of circulating human myeloid progenitor cells.Exp Hematol, vol. 8, no. 7, Aug. 1980, pp. 954–60.
Ross DD, Pollak A, Akman SA, Bachur NR. Diurnal variation of circulating human myeloid progenitor cells. Exp Hematol. 1980 Aug;8(7):954–960.
Journal cover image

Published In

Exp Hematol

ISSN

0301-472X

Publication Date

August 1980

Volume

8

Issue

7

Start / End Page

954 / 960

Location

Netherlands

Related Subject Headings

  • Neutrophils
  • Myeloid Progenitor Cells
  • Male
  • Macrophages
  • Immunology
  • Humans
  • Colony-Forming Units Assay
  • Circadian Rhythm
  • Cells, Cultured
  • 3201 Cardiovascular medicine and haematology