Skip to main content

Resistance to hydrogen peroxide associated with altered catalase mRNA stability in MCF7 breast cancer cells.

Publication ,  Journal Article
Akman, SA; Forrest, G; Chu, FF; Doroshow, JH
Published in: Biochim Biophys Acta
September 21, 1989

We have established a variant of the human breast cancer cell line MCF7, designated MCF7/H2O2, which is 5-fold resistant to H2O2 by clonogenic assay. The specific activity of the H2O2 disposal enzyme catalase was elevated 3-fold in MCF7/H2O2; activities of other antioxidant enzymes, including glutathione peroxidase and superoxide dismutase, were not increased. The steady-state level of catalase mRNA was only slightly elevated (approx. 1.6-fold) in MCF7/H2O2 cells; however, degradation of catalase mRNA was markedly retarded in MCF-7/H2O2 compared to MCF-7 (82% of catalase mRNA remained 24 h after inhibition of RNA synthesis by actinomycin D in MCF-7/H2O2 vs. 32% in MCF7). The degradation rates of superoxide dismutase mRNA and 28 S ribosomal RNA were not reduced in MCF-7/H2O2; however, the rate of degradation of another mRNA species, beta-actin, was also significantly decreased. These data suggest that resistance to H2O2 in MCF7/H2O2 cells is mediated by elevated catalase activity which can be explained by stabilization of certain mRNA species, including catalase mRNA.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

September 21, 1989

Volume

1009

Issue

1

Start / End Page

70 / 74

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Superoxide Dismutase
  • RNA, Messenger
  • Hydrogen Peroxide
  • Humans
  • Half-Life
  • Glutathione Peroxidase
  • Drug Resistance
  • Dactinomycin
  • Catalase
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Akman, S. A., Forrest, G., Chu, F. F., & Doroshow, J. H. (1989). Resistance to hydrogen peroxide associated with altered catalase mRNA stability in MCF7 breast cancer cells. Biochim Biophys Acta, 1009(1), 70–74. https://doi.org/10.1016/0167-4781(89)90080-8
Akman, S. A., G. Forrest, F. F. Chu, and J. H. Doroshow. “Resistance to hydrogen peroxide associated with altered catalase mRNA stability in MCF7 breast cancer cells.Biochim Biophys Acta 1009, no. 1 (September 21, 1989): 70–74. https://doi.org/10.1016/0167-4781(89)90080-8.
Akman SA, Forrest G, Chu FF, Doroshow JH. Resistance to hydrogen peroxide associated with altered catalase mRNA stability in MCF7 breast cancer cells. Biochim Biophys Acta. 1989 Sep 21;1009(1):70–4.
Akman, S. A., et al. “Resistance to hydrogen peroxide associated with altered catalase mRNA stability in MCF7 breast cancer cells.Biochim Biophys Acta, vol. 1009, no. 1, Sept. 1989, pp. 70–74. Pubmed, doi:10.1016/0167-4781(89)90080-8.
Akman SA, Forrest G, Chu FF, Doroshow JH. Resistance to hydrogen peroxide associated with altered catalase mRNA stability in MCF7 breast cancer cells. Biochim Biophys Acta. 1989 Sep 21;1009(1):70–74.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

September 21, 1989

Volume

1009

Issue

1

Start / End Page

70 / 74

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Superoxide Dismutase
  • RNA, Messenger
  • Hydrogen Peroxide
  • Humans
  • Half-Life
  • Glutathione Peroxidase
  • Drug Resistance
  • Dactinomycin
  • Catalase