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Molecular methods for the diagnosis of genital ulcer disease in a sexually transmitted disease clinic population in northern Thailand: predominance of herpes simplex virus infection.

Publication ,  Journal Article
Beyrer, C; Jitwatcharanan, K; Natpratan, C; Kaewvichit, R; Nelson, KE; Chen, CY; Weiss, JB; Morse, SA
Published in: J Infect Dis
July 1998

A multiplex polymerase chain reaction (M-PCR) assay that simultaneously detects the three major causes of genital ulcer disease (GUD), Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus, was used to evaluate swab specimens for 38 sequential patients with GUD at a Thai sexually transmitted disease clinic. Subjects received clinical diagnoses and syndromic treatment. Swab specimens for H. ducreyi cultures and M-PCR were obtained. No H. ducreyi cultures were positive. Of 38 M-PCR specimens, 31 (81.6%) were positive for HSV, 1 (2.3%) for both HSV and T. pallidum, and none for H. ducreyi or T. pallidum alone; 6 (15.8%) were negative for all 3 pathogens. Clinical diagnoses corresponded poorly to M-PCR findings; none of 5 suspected cases of chancroid were positive by M-PCR and none of 1 for syphilis, but 21 of 24 suspected herpes lesions were confirmed by M-PCR. Human immunodeficiency virus infection status was known for 24 of 38 subjects; 11 (45.8%) were seropositive, and all 11 had HSV by M-PCR. HSV appeared to be the most common pathogen overall.

Duke Scholars

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Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

July 1998

Volume

178

Issue

1

Start / End Page

243 / 246

Location

United States

Related Subject Headings

  • Ulcer
  • Treponema pallidum
  • Thailand
  • Syphilis
  • Simplexvirus
  • Reagent Kits, Diagnostic
  • Microbiology
  • Male
  • Humans
  • Herpes Genitalis
 

Citation

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Beyrer, C., Jitwatcharanan, K., Natpratan, C., Kaewvichit, R., Nelson, K. E., Chen, C. Y., … Morse, S. A. (1998). Molecular methods for the diagnosis of genital ulcer disease in a sexually transmitted disease clinic population in northern Thailand: predominance of herpes simplex virus infection. J Infect Dis, 178(1), 243–246. https://doi.org/10.1086/515603
Beyrer, C., K. Jitwatcharanan, C. Natpratan, R. Kaewvichit, K. E. Nelson, C. Y. Chen, J. B. Weiss, and S. A. Morse. “Molecular methods for the diagnosis of genital ulcer disease in a sexually transmitted disease clinic population in northern Thailand: predominance of herpes simplex virus infection.J Infect Dis 178, no. 1 (July 1998): 243–46. https://doi.org/10.1086/515603.
Beyrer C, Jitwatcharanan K, Natpratan C, Kaewvichit R, Nelson KE, Chen CY, Weiss JB, Morse SA. Molecular methods for the diagnosis of genital ulcer disease in a sexually transmitted disease clinic population in northern Thailand: predominance of herpes simplex virus infection. J Infect Dis. 1998 Jul;178(1):243–246.
Journal cover image

Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

July 1998

Volume

178

Issue

1

Start / End Page

243 / 246

Location

United States

Related Subject Headings

  • Ulcer
  • Treponema pallidum
  • Thailand
  • Syphilis
  • Simplexvirus
  • Reagent Kits, Diagnostic
  • Microbiology
  • Male
  • Humans
  • Herpes Genitalis