Subcellular localization of the human proto-oncogene protein DEK.
Recent data revealed that DEK associates with splicing complexes through interactions mediated by serine/arginine-repeat proteins. However, the DEK protein has also been shown to change the topology of DNA in chromatin in vitro. This could indicate that the DEK protein resides on cellular chromatin. To investigate the in vivo localization of DEK, we performed cell fractionation studies, immunolabeling, and micrococcal nuclease digestion analysis. Most of the DEK protein was found to be released by DNase treatment of nuclei, and only a small amount by treatment with RNase. Furthermore, micrococcal nuclease digestion of nuclei followed by glycerol gradient sedimentation revealed that DEK co-sedimentates with oligonucleosomes, clearly demonstrating that DEK is associated with chromatin in vivo. Additional chromatin fractionation studies, based on the different accessibilities to micrococcal nuclease, showed that DEK is associated both with extended, genetically active and more densely organized, inactive chromatin. We found no significant change in the amount and localization of DEK in cells that synchronously traversed the cell cycle. In summary these data demonstrate that the major portion of DEK is associated with chromatin in vivo and suggest that it might play a role in chromatin architecture.
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Related Subject Headings
- Proto-Oncogene Mas
- Oncogene Proteins
- Humans
- Chromatin
- Cell Cycle
- Biochemistry & Molecular Biology
- 34 Chemical sciences
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 11 Medical and Health Sciences
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Proto-Oncogene Mas
- Oncogene Proteins
- Humans
- Chromatin
- Cell Cycle
- Biochemistry & Molecular Biology
- 34 Chemical sciences
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 11 Medical and Health Sciences