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Suppressyn localization and dynamic expression patterns in primary human tissues support a physiologic role in human placentation.

Publication ,  Journal Article
Sugimoto, J; Schust, DJ; Kinjo, T; Aoki, Y; Jinno, Y; Kudo, Y
Published in: Sci Rep
December 20, 2019

We previously identified suppressyn (SUPYN), a placental protein that negatively regulates the cell fusion essential for trophoblast syncytialization via binding to the trophoblast receptor for syncytin-1, ASCT2, and hypothesized that SUPYN may thereby regulate cell-cell fusion in the placenta. Here, we redefine in vivo SUPYN localization using specific monoclonal antibodies in a rare early placental sample, showing SUPYN localization in villous and extravillous trophoblast subtypes, the decidua and even in placental debris in the maternal vasculature. In human trophoblast cell lines, we show SUPYN alters ASCT2 glycosylation within the secretory pathway and that this binding is associated with inhibition of cell fusion. Using newly-optimized trophoblast isolation protocols that allow tracking of ex vivo cell fusion, we present transcription and translation dynamics of fusion-related proteins over 96 hours in culture and the effects of changes in ambient oxygen levels on these processes. We report converse syncytin-1 and SUPYN transcriptional and translational responses to surrounding oxygen concentrations that suggest both are important in the effects of hypoxia and hyperoxia on placental syncytialization. Our results suggest that SUPYN's anti-fusogenic properties may be exerted at several sites in the maternal body and its dysregulation may be associated with diseases of abnormal placentation.

Duke Scholars

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

December 20, 2019

Volume

9

Issue

1

Start / End Page

19502

Location

England

Related Subject Headings

  • Young Adult
  • Trophoblasts
  • Pregnancy Proteins
  • Pregnancy
  • Placentation
  • Minor Histocompatibility Antigens
  • Humans
  • Glycosylation
  • Gene Products, env
  • Female
 

Citation

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Sugimoto, J., Schust, D. J., Kinjo, T., Aoki, Y., Jinno, Y., & Kudo, Y. (2019). Suppressyn localization and dynamic expression patterns in primary human tissues support a physiologic role in human placentation. Sci Rep, 9(1), 19502. https://doi.org/10.1038/s41598-019-55933-x
Sugimoto, Jun, Danny J. Schust, Tadatsugu Kinjo, Yoichi Aoki, Yoshihiro Jinno, and Yoshiki Kudo. “Suppressyn localization and dynamic expression patterns in primary human tissues support a physiologic role in human placentation.Sci Rep 9, no. 1 (December 20, 2019): 19502. https://doi.org/10.1038/s41598-019-55933-x.
Sugimoto J, Schust DJ, Kinjo T, Aoki Y, Jinno Y, Kudo Y. Suppressyn localization and dynamic expression patterns in primary human tissues support a physiologic role in human placentation. Sci Rep. 2019 Dec 20;9(1):19502.
Sugimoto, Jun, et al. “Suppressyn localization and dynamic expression patterns in primary human tissues support a physiologic role in human placentation.Sci Rep, vol. 9, no. 1, Dec. 2019, p. 19502. Pubmed, doi:10.1038/s41598-019-55933-x.
Sugimoto J, Schust DJ, Kinjo T, Aoki Y, Jinno Y, Kudo Y. Suppressyn localization and dynamic expression patterns in primary human tissues support a physiologic role in human placentation. Sci Rep. 2019 Dec 20;9(1):19502.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

December 20, 2019

Volume

9

Issue

1

Start / End Page

19502

Location

England

Related Subject Headings

  • Young Adult
  • Trophoblasts
  • Pregnancy Proteins
  • Pregnancy
  • Placentation
  • Minor Histocompatibility Antigens
  • Humans
  • Glycosylation
  • Gene Products, env
  • Female