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Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be?

Publication ,  Journal Article
Roberts, RM; Loh, KM; Amita, M; Bernardo, AS; Adachi, K; Alexenko, AP; Schust, DJ; Schulz, LC; Telugu, BPVL; Ezashi, T; Pedersen, RA
Published in: Reproduction
May 2014

It is imperative to unveil the full range of differentiated cell types into which human pluripotent stem cells (hPSCs) can develop. The need is twofold: it will delimit the therapeutic utility of these stem cells and is necessary to place their position accurately in the developmental hierarchy of lineage potential. Accumulated evidence suggested that hPSC could develop in vitro into an extraembryonic lineage (trophoblast (TB)) that is typically inaccessible to pluripotent embryonic cells during embryogenesis. However, whether these differentiated cells are truly authentic TB has been challenged. In this debate, we present a case for and a case against TB differentiation from hPSCs. By analogy to other differentiation systems, our debate is broadly applicable, as it articulates higher and more challenging standards for judging whether a given cell type has been genuinely produced from hPSC differentiation.

Duke Scholars

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Published In

Reproduction

DOI

EISSN

1741-7899

Publication Date

May 2014

Volume

147

Issue

5

Start / End Page

D1 / 12

Location

England

Related Subject Headings

  • Trophoblasts
  • Pregnancy
  • Placenta
  • Obstetrics & Reproductive Medicine
  • Morphogenesis
  • In Vitro Techniques
  • Humans
  • Female
  • Embryonic Stem Cells
  • Cells, Cultured
 

Citation

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Roberts, R. M., Loh, K. M., Amita, M., Bernardo, A. S., Adachi, K., Alexenko, A. P., … Pedersen, R. A. (2014). Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be? Reproduction, 147(5), D1-12. https://doi.org/10.1530/REP-14-0080
Roberts, R Michael, Kyle M. Loh, Mitsuyoshi Amita, Andreia S. Bernardo, Katsuyuki Adachi, Andrei P. Alexenko, Danny J. Schust, et al. “Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be?Reproduction 147, no. 5 (May 2014): D1-12. https://doi.org/10.1530/REP-14-0080.
Roberts RM, Loh KM, Amita M, Bernardo AS, Adachi K, Alexenko AP, et al. Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be? Reproduction. 2014 May;147(5):D1-12.
Roberts, R. Michael, et al. “Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be?Reproduction, vol. 147, no. 5, May 2014, pp. D1-12. Pubmed, doi:10.1530/REP-14-0080.
Roberts RM, Loh KM, Amita M, Bernardo AS, Adachi K, Alexenko AP, Schust DJ, Schulz LC, Telugu BPVL, Ezashi T, Pedersen RA. Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be? Reproduction. 2014 May;147(5):D1-12.

Published In

Reproduction

DOI

EISSN

1741-7899

Publication Date

May 2014

Volume

147

Issue

5

Start / End Page

D1 / 12

Location

England

Related Subject Headings

  • Trophoblasts
  • Pregnancy
  • Placenta
  • Obstetrics & Reproductive Medicine
  • Morphogenesis
  • In Vitro Techniques
  • Humans
  • Female
  • Embryonic Stem Cells
  • Cells, Cultured