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CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.

Publication ,  Journal Article
Kawana, K; Quayle, AJ; Ficarra, M; Ibana, JA; Shen, L; Kawana, Y; Yang, H; Marrero, L; Yavagal, S; Greene, SJ; Zhang, Y-X; Pyles, RB ...
Published in: J Biol Chem
March 9, 2007

Chlamydia trachomatis is an obligate intracellular pathogen that can persist in the urogenital tract. Mechanisms by which C. trachomatis evades clearance by host innate immune responses are poorly described. CD1d is MHC-like, is expressed by epithelial cells, and can signal innate immune responses by NK and NKT cells. Here we demonstrate that C. trachomatis infection down-regulates surface-expressed CD1d in human penile urethral epithelial cells through proteasomal degradation. A chlamydial proteasome-like activity factor (CPAF) interacts with the CD1d heavy chain, and CPAF-associated CD1d heavy chain is then ubiquitinated and directed along two distinct proteolytic pathways. The degradation of immature glycosylated CD1d was blocked by the proteasome inhibitor lactacystin but not by MG132, indicating that degradation was not via the conventional proteasome. In contrast, the degradation of non-glycosylated CD1d was blocked by lactacystin and MG132, consistent with conventional cellular cytosolic degradation of N-linked glycoproteins. Immunofluorescent microscopy confirmed the interruption of CD1d trafficking to the cell surface, and the dislocation of CD1d heavy chains into both the cellular cytosol and the chlamydial inclusion along with cytosolic CPAF. C. trachomatis targeted CD1d toward two distinct proteolytic pathways. Decreased CD1d surface expression may help C. trachomatis evade detection by innate immune cells and may promote C. trachomatis persistence.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 9, 2007

Volume

282

Issue

10

Start / End Page

7368 / 7375

Location

United States

Related Subject Headings

  • Proteasome Endopeptidase Complex
  • Molecular Sequence Data
  • Microscopy, Fluorescence
  • Male
  • Immunoprecipitation
  • Immunity, Innate
  • Humans
  • Epithelial Cells
  • Chlamydia trachomatis
  • Cell Line
 

Citation

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Kawana, K., Quayle, A. J., Ficarra, M., Ibana, J. A., Shen, L., Kawana, Y., … Schust, D. J. (2007). CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity. J Biol Chem, 282(10), 7368–7375. https://doi.org/10.1074/jbc.M610754200
Kawana, Kei, Alison J. Quayle, Mercedes Ficarra, Joyce A. Ibana, Li Shen, Yukiko Kawana, Huixia Yang, et al. “CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.J Biol Chem 282, no. 10 (March 9, 2007): 7368–75. https://doi.org/10.1074/jbc.M610754200.
Kawana K, Quayle AJ, Ficarra M, Ibana JA, Shen L, Kawana Y, et al. CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity. J Biol Chem. 2007 Mar 9;282(10):7368–75.
Kawana, Kei, et al. “CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity.J Biol Chem, vol. 282, no. 10, Mar. 2007, pp. 7368–75. Pubmed, doi:10.1074/jbc.M610754200.
Kawana K, Quayle AJ, Ficarra M, Ibana JA, Shen L, Kawana Y, Yang H, Marrero L, Yavagal S, Greene SJ, Zhang Y-X, Pyles RB, Blumberg RS, Schust DJ. CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity. J Biol Chem. 2007 Mar 9;282(10):7368–7375.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 9, 2007

Volume

282

Issue

10

Start / End Page

7368 / 7375

Location

United States

Related Subject Headings

  • Proteasome Endopeptidase Complex
  • Molecular Sequence Data
  • Microscopy, Fluorescence
  • Male
  • Immunoprecipitation
  • Immunity, Innate
  • Humans
  • Epithelial Cells
  • Chlamydia trachomatis
  • Cell Line