Skip to main content
Journal cover image

Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2?

Publication ,  Journal Article
Erickson, HP; Corbin Goodman, L
Published in: Biochemistry
January 17, 2023

The trimeric spike protein of SARS-CoV-2 has been targeted by antibody mimics that bind near or at the receptor-binding domain to neutralize the virus. Several independent studies have reported enhanced binding avidity for dimers and trimers, where binding domains are connected by short peptides. The enhanced avidity of the multivalent constructs was attributed to their simultaneously binding two or three sites within a single spike trimer. We argue here that the 15-20 amino acid peptide linkers, when considered as worm-like-chains, are too short to span the binding sites within a single spike. The enhanced avidity of the multivalent constructs may be explained by a rebinding mechanism, which does not involve multivalent binding.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biochemistry

DOI

EISSN

1520-4995

Publication Date

January 17, 2023

Volume

62

Issue

2

Start / End Page

163 / 168

Location

United States

Related Subject Headings

  • Spike Glycoprotein, Coronavirus
  • SARS-CoV-2
  • Protein Binding
  • Peptides
  • Humans
  • COVID-19
  • Biochemistry & Molecular Biology
  • Binding Sites
  • 3404 Medicinal and biomolecular chemistry
  • 3205 Medical biochemistry and metabolomics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Erickson, H. P., & Corbin Goodman, L. (2023). Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2? Biochemistry, 62(2), 163–168. https://doi.org/10.1021/acs.biochem.2c00291
Erickson, Harold P., and Lauren Corbin Goodman. “Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2?Biochemistry 62, no. 2 (January 17, 2023): 163–68. https://doi.org/10.1021/acs.biochem.2c00291.
Erickson, Harold P., and Lauren Corbin Goodman. “Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2?Biochemistry, vol. 62, no. 2, Jan. 2023, pp. 163–68. Pubmed, doi:10.1021/acs.biochem.2c00291.
Journal cover image

Published In

Biochemistry

DOI

EISSN

1520-4995

Publication Date

January 17, 2023

Volume

62

Issue

2

Start / End Page

163 / 168

Location

United States

Related Subject Headings

  • Spike Glycoprotein, Coronavirus
  • SARS-CoV-2
  • Protein Binding
  • Peptides
  • Humans
  • COVID-19
  • Biochemistry & Molecular Biology
  • Binding Sites
  • 3404 Medicinal and biomolecular chemistry
  • 3205 Medical biochemistry and metabolomics