Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2?
Publication
, Journal Article
Erickson, HP; Corbin Goodman, L
Published in: Biochemistry
January 17, 2023
The trimeric spike protein of SARS-CoV-2 has been targeted by antibody mimics that bind near or at the receptor-binding domain to neutralize the virus. Several independent studies have reported enhanced binding avidity for dimers and trimers, where binding domains are connected by short peptides. The enhanced avidity of the multivalent constructs was attributed to their simultaneously binding two or three sites within a single spike trimer. We argue here that the 15-20 amino acid peptide linkers, when considered as worm-like-chains, are too short to span the binding sites within a single spike. The enhanced avidity of the multivalent constructs may be explained by a rebinding mechanism, which does not involve multivalent binding.
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Published In
Biochemistry
DOI
EISSN
1520-4995
Publication Date
January 17, 2023
Volume
62
Issue
2
Start / End Page
163 / 168
Location
United States
Related Subject Headings
- Spike Glycoprotein, Coronavirus
- SARS-CoV-2
- Protein Binding
- Peptides
- Humans
- COVID-19
- Biochemistry & Molecular Biology
- Binding Sites
- 3404 Medicinal and biomolecular chemistry
- 3205 Medical biochemistry and metabolomics
Citation
APA
Chicago
ICMJE
MLA
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Erickson, H. P., & Corbin Goodman, L. (2023). Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2? Biochemistry, 62(2), 163–168. https://doi.org/10.1021/acs.biochem.2c00291
Erickson, Harold P., and Lauren Corbin Goodman. “Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2?” Biochemistry 62, no. 2 (January 17, 2023): 163–68. https://doi.org/10.1021/acs.biochem.2c00291.
Erickson HP, Corbin Goodman L. Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2? Biochemistry. 2023 Jan 17;62(2):163–8.
Erickson, Harold P., and Lauren Corbin Goodman. “Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2?” Biochemistry, vol. 62, no. 2, Jan. 2023, pp. 163–68. Pubmed, doi:10.1021/acs.biochem.2c00291.
Erickson HP, Corbin Goodman L. Recently Designed Multivalent Spike Binders Cannot Bind Multivalently─How Do They Achieve Enhanced Avidity to SARS-CoV-2? Biochemistry. 2023 Jan 17;62(2):163–168.
Published In
Biochemistry
DOI
EISSN
1520-4995
Publication Date
January 17, 2023
Volume
62
Issue
2
Start / End Page
163 / 168
Location
United States
Related Subject Headings
- Spike Glycoprotein, Coronavirus
- SARS-CoV-2
- Protein Binding
- Peptides
- Humans
- COVID-19
- Biochemistry & Molecular Biology
- Binding Sites
- 3404 Medicinal and biomolecular chemistry
- 3205 Medical biochemistry and metabolomics