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Detection of m6A in single cultured cells using scDART-seq.

Publication ,  Journal Article
Tegowski, M; Meyer, KD
Published in: STAR Protoc
September 16, 2022

Most techniques for mapping m6A-methylated RNAs transcriptome-wide require large amounts of RNA and have been limited to bulk cells and tissues. Here, we provide a detailed protocol for the identification of m6A sites in single HEK293T cells using single-cell DART-seq (scDART-seq). The protocol details how to generate cell lines with inducible expression of the APOBEC1-YTH transgene and the use of important controls for minimizing false positives. We also describe the bioinformatic analysis to identify m6A sites. For complete details on the use and execution of this protocol, please refer to Tegowski et al. (2022).

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Published In

STAR Protoc

DOI

EISSN

2666-1667

Publication Date

September 16, 2022

Volume

3

Issue

3

Start / End Page

101646

Location

United States

Related Subject Headings

  • Transcriptome
  • Sequence Analysis, RNA
  • RNA
  • Humans
  • High-Throughput Nucleotide Sequencing
  • HEK293 Cells
  • APOBEC-1 Deaminase
 

Citation

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Tegowski, M., & Meyer, K. D. (2022). Detection of m6A in single cultured cells using scDART-seq. STAR Protoc, 3(3), 101646. https://doi.org/10.1016/j.xpro.2022.101646
Tegowski, Matthew, and Kate D. Meyer. “Detection of m6A in single cultured cells using scDART-seq.STAR Protoc 3, no. 3 (September 16, 2022): 101646. https://doi.org/10.1016/j.xpro.2022.101646.
Tegowski M, Meyer KD. Detection of m6A in single cultured cells using scDART-seq. STAR Protoc. 2022 Sep 16;3(3):101646.
Tegowski, Matthew, and Kate D. Meyer. “Detection of m6A in single cultured cells using scDART-seq.STAR Protoc, vol. 3, no. 3, Sept. 2022, p. 101646. Pubmed, doi:10.1016/j.xpro.2022.101646.
Tegowski M, Meyer KD. Detection of m6A in single cultured cells using scDART-seq. STAR Protoc. 2022 Sep 16;3(3):101646.
Journal cover image

Published In

STAR Protoc

DOI

EISSN

2666-1667

Publication Date

September 16, 2022

Volume

3

Issue

3

Start / End Page

101646

Location

United States

Related Subject Headings

  • Transcriptome
  • Sequence Analysis, RNA
  • RNA
  • Humans
  • High-Throughput Nucleotide Sequencing
  • HEK293 Cells
  • APOBEC-1 Deaminase