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pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion.

Publication ,  Journal Article
Chang, AL; Kim, Y; Seitz, AP; Schuster, RM; Pritts, TA
Published in: J Surg Res
May 15, 2017

BACKGROUND: Prolonged storage of packed red blood cells (pRBCs) induces a series of harmful biochemical and metabolic changes known as the RBC storage lesion. RBCs are currently stored in an acidic storage solution, but the effect of pH on the RBC storage lesion is unknown. We investigated the effect of modulation of storage pH on the RBC storage lesion and on erythrocyte survival after transfusion. METHODS: Murine pRBCs were stored in Additive Solution-3 (AS3) under standard conditions (pH, 5.8), acidic AS3 (pH, 4.5), or alkalinized AS3 (pH, 8.5). pRBC units were analyzed at the end of the storage period. Several components of the storage lesion were measured, including cell-free hemoglobin, microparticle production, phosphatidylserine externalization, lactate accumulation, and byproducts of lipid peroxidation. Carboxyfluorescein-labeled erythrocytes were transfused into healthy mice to determine cell survival. RESULTS: Compared with pRBCs stored in standard AS3, those stored in alkaline solution exhibited decreased hemolysis, phosphatidylserine externalization, microparticle production, and lipid peroxidation. Lactate levels were greater after storage in alkaline conditions, suggesting that these pRBCs remained more metabolically viable. Storage in acidic AS3 accelerated erythrocyte deterioration. Compared with standard AS3 storage, circulating half-life of cells was increased by alkaline storage but decreased in acidic conditions. CONCLUSIONS: Storage pH significantly affects the quality of stored RBCs and cell survival after transfusion. Current erythrocyte storage solutions may benefit from refinements in pH levels.

Duke Scholars

Published In

J Surg Res

DOI

EISSN

1095-8673

Publication Date

May 15, 2017

Volume

212

Start / End Page

54 / 59

Location

United States

Related Subject Headings

  • Surgery
  • Preservatives, Pharmaceutical
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Hydrogen-Ion Concentration
  • Erythrocytes
  • Erythrocyte Transfusion
  • Cell Survival
  • Blood Preservation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Chang, A. L., Kim, Y., Seitz, A. P., Schuster, R. M., & Pritts, T. A. (2017). pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion. J Surg Res, 212, 54–59. https://doi.org/10.1016/j.jss.2016.12.025
Chang, Alex L., Young Kim, Aaron P. Seitz, Rebecca M. Schuster, and Timothy A. Pritts. “pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion.J Surg Res 212 (May 15, 2017): 54–59. https://doi.org/10.1016/j.jss.2016.12.025.
Chang AL, Kim Y, Seitz AP, Schuster RM, Pritts TA. pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion. J Surg Res. 2017 May 15;212:54–9.
Chang, Alex L., et al. “pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion.J Surg Res, vol. 212, May 2017, pp. 54–59. Pubmed, doi:10.1016/j.jss.2016.12.025.
Chang AL, Kim Y, Seitz AP, Schuster RM, Pritts TA. pH modulation ameliorates the red blood cell storage lesion in a murine model of transfusion. J Surg Res. 2017 May 15;212:54–59.
Journal cover image

Published In

J Surg Res

DOI

EISSN

1095-8673

Publication Date

May 15, 2017

Volume

212

Start / End Page

54 / 59

Location

United States

Related Subject Headings

  • Surgery
  • Preservatives, Pharmaceutical
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Hydrogen-Ion Concentration
  • Erythrocytes
  • Erythrocyte Transfusion
  • Cell Survival
  • Blood Preservation