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Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects

Bacterial detection methods

Publication ,  Chapter
Shaz, BH
June 8, 2009

This chapter focuses on the methods for detection of bacterial contamination of blood products and with emphasis on platelet products. Various methods are described for avoiding bacterial contamination such as donor screening, methods to reduce bacterial contamination and methods to inhibit or inactivate bacteria. In order to reduce the occurrence of bacterial contamination, the use of a diversion pouch to eliminate skin bacteria. Cold storage of the platelet inhibits or inactivates bacteria and decrease bacterial growth and reduces the risk for septic transfusion reactions. Culture-based methods are mentioned to detect contaminated products and two culture-based systems are described. Some other detection methods performed just prior to product release include immunoassay, staining and metabolic detection methods. Immunoassay is a FDA approved bacterial detection method for quality control of leukoreduced apheresis platelet products. The staining methods used for detection of bacteria in platelet products are gram stain, accrine orange and Wright stain.

Duke Scholars

DOI

ISBN

9780123744326

Publication Date

June 8, 2009

Start / End Page

83 / 86
 

Citation

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Shaz, B. H. (2009). Bacterial detection methods. In Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects (pp. 83–86). https://doi.org/10.1016/B978-0-12-374432-6.00017-8
Shaz, B. H. “Bacterial detection methods.” In Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects, 83–86, 2009. https://doi.org/10.1016/B978-0-12-374432-6.00017-8.
Shaz BH. Bacterial detection methods. In: Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects. 2009. p. 83–6.
Shaz, B. H. “Bacterial detection methods.” Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects, 2009, pp. 83–86. Scopus, doi:10.1016/B978-0-12-374432-6.00017-8.
Shaz BH. Bacterial detection methods. Transfusion Medicine and Hemostasis: Clinical and Laboratory Aspects. 2009. p. 83–86.
Journal cover image

DOI

ISBN

9780123744326

Publication Date

June 8, 2009

Start / End Page

83 / 86