Skip to main content

(New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples.

Publication ,  Journal Article
Jenks, JD; Spiess, B; Buchheidt, D; Hoenigl, M
Published in: Curr Fungal Infect Rep
September 2019

PURPOSE OF REVIEW: The incidence of invasive aspergillosis has increased substantially over the past few decades, accompanied by a change in susceptibility patterns of Aspergillus fumigatus with increasing resistance observed against triazole antifungals, including voriconazole and isavuconazole, the most commonly used antifungal agents for the disease. Culture-based methods for determining triazole resistance are still the gold standard but are time consuming and lack sensitivity. We sought to provide an update on non-culture-based methods for detecting resistance patterns to Aspergillus. RECENT FINDINGS: New molecular-based approaches for detecting triazole resistance to Aspergillus, real-time polymerase chain reaction (PCR) to detect mutations to the Cyp51A protein, have been developed which are able to detect most triazole-resistant A. fumigatus strains in patients with invasive aspergillosis. SUMMARY: Over the last few years, a number of non-culture-based methods for molecular detection of Aspergillus triazole resistance have been developed that may overcome some of the limitations of culture. These molecular methods are therefore of high epidemiological and clinical relevance, mainly in immunocompromised patients with hematological malignancies, where culture has particularly limited sensitivity. These assays are now able to detect most triazole-resistant Aspergillus fumigatus strains. Given that resistance rates vary, clinical utility for these assays still depends on regional resistance patterns.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Curr Fungal Infect Rep

DOI

ISSN

1936-3761

Publication Date

September 2019

Volume

13

Issue

3

Start / End Page

129 / 136

Location

United States

Related Subject Headings

  • 3207 Medical microbiology
  • 3202 Clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jenks, J. D., Spiess, B., Buchheidt, D., & Hoenigl, M. (2019). (New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples. Curr Fungal Infect Rep, 13(3), 129–136. https://doi.org/10.1007/s12281-019-00342-w
Jenks, Jeffrey D., Birgit Spiess, Dieter Buchheidt, and Martin Hoenigl. “(New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples.Curr Fungal Infect Rep 13, no. 3 (September 2019): 129–36. https://doi.org/10.1007/s12281-019-00342-w.
Jenks JD, Spiess B, Buchheidt D, Hoenigl M. (New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples. Curr Fungal Infect Rep. 2019 Sep;13(3):129–36.
Jenks, Jeffrey D., et al. “(New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples.Curr Fungal Infect Rep, vol. 13, no. 3, Sept. 2019, pp. 129–36. Pubmed, doi:10.1007/s12281-019-00342-w.
Jenks JD, Spiess B, Buchheidt D, Hoenigl M. (New) Methods for Detection of Aspergillus fumigatus Resistance in Clinical Samples. Curr Fungal Infect Rep. 2019 Sep;13(3):129–136.

Published In

Curr Fungal Infect Rep

DOI

ISSN

1936-3761

Publication Date

September 2019

Volume

13

Issue

3

Start / End Page

129 / 136

Location

United States

Related Subject Headings

  • 3207 Medical microbiology
  • 3202 Clinical sciences