Protocol to measure endotoxin from opaque tissues in mice using an optimized kinetic limulus amebocyte lysate assay.
Publication
, Journal Article
Ceasrine, AM; Green, LA; Bilbo, SD
Published in: STAR protocols
December 2022
Endotoxin accumulation has been widely noted in several pathologies ranging from metabolic dysregulation to bacterial infection. Using limulus amebocyte lysate (LAL) assays to detect endotoxin load has been the only reliable way to assess endotoxin accumulation, but assays optimized for detection in opaque tissues are still lacking. We optimized a sensitive Kinetic LAL assay for endotoxin detection from murine tissues. In this protocol, we describe tissue collection and homogenization, followed by the procedure to run the assay and data analysis. For complete details on the use and execution of this protocol, please refer to Ceasrine et al. (2022).
Duke Scholars
Published In
STAR protocols
DOI
EISSN
2666-1667
ISSN
2666-1667
Publication Date
December 2022
Volume
3
Issue
4
Start / End Page
101669
Related Subject Headings
- Mice
- Limulus Test
- Kinetics
- Horseshoe Crabs
- Endotoxins
- Biological Assay
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Ceasrine, A. M., Green, L. A., & Bilbo, S. D. (2022). Protocol to measure endotoxin from opaque tissues in mice using an optimized kinetic limulus amebocyte lysate assay. STAR Protocols, 3(4), 101669. https://doi.org/10.1016/j.xpro.2022.101669
Ceasrine, Alexis M., Lauren A. Green, and Staci D. Bilbo. “Protocol to measure endotoxin from opaque tissues in mice using an optimized kinetic limulus amebocyte lysate assay.” STAR Protocols 3, no. 4 (December 2022): 101669. https://doi.org/10.1016/j.xpro.2022.101669.
Ceasrine AM, Green LA, Bilbo SD. Protocol to measure endotoxin from opaque tissues in mice using an optimized kinetic limulus amebocyte lysate assay. STAR protocols. 2022 Dec;3(4):101669.
Ceasrine, Alexis M., et al. “Protocol to measure endotoxin from opaque tissues in mice using an optimized kinetic limulus amebocyte lysate assay.” STAR Protocols, vol. 3, no. 4, Dec. 2022, p. 101669. Epmc, doi:10.1016/j.xpro.2022.101669.
Ceasrine AM, Green LA, Bilbo SD. Protocol to measure endotoxin from opaque tissues in mice using an optimized kinetic limulus amebocyte lysate assay. STAR protocols. 2022 Dec;3(4):101669.
Published In
STAR protocols
DOI
EISSN
2666-1667
ISSN
2666-1667
Publication Date
December 2022
Volume
3
Issue
4
Start / End Page
101669
Related Subject Headings
- Mice
- Limulus Test
- Kinetics
- Horseshoe Crabs
- Endotoxins
- Biological Assay
- Animals