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CovR activation of the dipeptide permease promoter (PdppA) in Group A Streptococcus.

Publication ,  Journal Article
Gusa, AA; Froehlich, BJ; Desai, D; Stringer, V; Scott, JR
Published in: J Bacteriol
February 2007

CovR, the two-component response regulator of Streptococcus pyogenes (group A streptococcus [GAS]) directly or indirectly represses about 15% of the genome, including genes encoding many virulence factors and itself. Transcriptome analyses also showed that some genes are activated by CovR. We asked whether the regulation by CovR of one of these genes, dppA, the first gene in an operon encoding a dipeptide permease, is direct or indirect. Direct regulation by CovR was suggested by the presence of five CovR consensus binding sequences (CBs) near the putative promoter. In this study, we identified the 5' end of the dppA transcript synthesized in vivo and showed that the start of dppA transcription in vitro is the same. We found that CovR binds specifically to the dppA promoter region (PdppA) in vitro with an affinity similar to that at which it binds to other CovR-regulated promoters. Disruption of any of the five CBs by a substitution of GG for TT inhibited CovR binding to that site in vitro, and binding at two of the CBs appeared cooperative. In vivo, CovR activation of transcription was not affected by individual mutations of any of the four CBs that we could study. This suggests that the binding sites are redundant in vivo. In vitro, CovR did not activate transcription from PdppA in experiments using purified GAS RNA polymerase and either linear or supercoiled DNA template. Therefore, we propose that in vivo, CovR may interfere with the binding of a repressor of PdppA.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

February 2007

Volume

189

Issue

4

Start / End Page

1407 / 1416

Location

United States

Related Subject Headings

  • Streptococcus pyogenes
  • Repressor Proteins
  • Protein Binding
  • Promoter Regions, Genetic
  • Mutation
  • Molecular Sequence Data
  • Microbiology
  • Membrane Transport Proteins
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Bacterial
 

Citation

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Gusa, A. A., Froehlich, B. J., Desai, D., Stringer, V., & Scott, J. R. (2007). CovR activation of the dipeptide permease promoter (PdppA) in Group A Streptococcus. J Bacteriol, 189(4), 1407–1416. https://doi.org/10.1128/JB.01036-06
Gusa, Asiya A., Barbara J. Froehlich, Devak Desai, Virginia Stringer, and June R. Scott. “CovR activation of the dipeptide permease promoter (PdppA) in Group A Streptococcus.J Bacteriol 189, no. 4 (February 2007): 1407–16. https://doi.org/10.1128/JB.01036-06.
Gusa AA, Froehlich BJ, Desai D, Stringer V, Scott JR. CovR activation of the dipeptide permease promoter (PdppA) in Group A Streptococcus. J Bacteriol. 2007 Feb;189(4):1407–16.
Gusa, Asiya A., et al. “CovR activation of the dipeptide permease promoter (PdppA) in Group A Streptococcus.J Bacteriol, vol. 189, no. 4, Feb. 2007, pp. 1407–16. Pubmed, doi:10.1128/JB.01036-06.
Gusa AA, Froehlich BJ, Desai D, Stringer V, Scott JR. CovR activation of the dipeptide permease promoter (PdppA) in Group A Streptococcus. J Bacteriol. 2007 Feb;189(4):1407–1416.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

February 2007

Volume

189

Issue

4

Start / End Page

1407 / 1416

Location

United States

Related Subject Headings

  • Streptococcus pyogenes
  • Repressor Proteins
  • Protein Binding
  • Promoter Regions, Genetic
  • Mutation
  • Molecular Sequence Data
  • Microbiology
  • Membrane Transport Proteins
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Bacterial