A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults.
Publication
, Journal Article
Smith, JJ; Kenny, IW; Wolff, C; Cray, R; Kumar, A; Sherwood, DR; Matus, DQ
Published in: Frontiers in cell and developmental biology
January 2022
Light sheet fluorescence microscopy (LSFM) has become a method of choice for live imaging because of its fast acquisition and reduced photobleaching and phototoxicity. Despite the strengths and growing availability of LSFM systems, no generalized LSFM mounting protocol has been adapted for live imaging of post-embryonic stages of C. elegans. A major challenge has been to develop methods to limit animal movement using a mounting media that matches the refractive index of the optical system. Here, we describe a simple mounting and immobilization protocol using a refractive-index matched UV-curable hydrogel within fluorinated ethylene propylene (FEP) tubes for efficient and reliable imaging of larval and adult C. elegans stages.
Duke Scholars
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Published In
Frontiers in cell and developmental biology
DOI
EISSN
2296-634X
ISSN
2296-634X
Publication Date
January 2022
Volume
10
Start / End Page
1012820
Related Subject Headings
- 32 Biomedical and clinical sciences
- 31 Biological sciences
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Smith, J. J., Kenny, I. W., Wolff, C., Cray, R., Kumar, A., Sherwood, D. R., & Matus, D. Q. (2022). A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults. Frontiers in Cell and Developmental Biology, 10, 1012820. https://doi.org/10.3389/fcell.2022.1012820
Smith, Jayson J., Isabel W. Kenny, Carsten Wolff, Rachel Cray, Abhishek Kumar, David R. Sherwood, and David Q. Matus. “A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults.” Frontiers in Cell and Developmental Biology 10 (January 2022): 1012820. https://doi.org/10.3389/fcell.2022.1012820.
Smith JJ, Kenny IW, Wolff C, Cray R, Kumar A, Sherwood DR, et al. A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults. Frontiers in cell and developmental biology. 2022 Jan;10:1012820.
Smith, Jayson J., et al. “A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults.” Frontiers in Cell and Developmental Biology, vol. 10, Jan. 2022, p. 1012820. Epmc, doi:10.3389/fcell.2022.1012820.
Smith JJ, Kenny IW, Wolff C, Cray R, Kumar A, Sherwood DR, Matus DQ. A light sheet fluorescence microscopy protocol for Caenorhabditis elegans larvae and adults. Frontiers in cell and developmental biology. 2022 Jan;10:1012820.
Published In
Frontiers in cell and developmental biology
DOI
EISSN
2296-634X
ISSN
2296-634X
Publication Date
January 2022
Volume
10
Start / End Page
1012820
Related Subject Headings
- 32 Biomedical and clinical sciences
- 31 Biological sciences